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长链非编码 RNA CARMN、LUCAT1、SMILR 和 MALAT1 在胸主动脉瘤中的作用：临床样本中生物标志物的验证。

Long Noncoding RNAs CARMN, LUCAT1, SMILR, and MALAT1 in Thoracic Aortic Aneurysm: Validation of Biomarkers in Clinical Samples.

机构信息

Institute of Cardiology, Lithuanian University of Health Sciences, Sukilėlių pr. 15, Kaunas LT-50103, Lithuania.

Department of Cardiac, Thoracic and Vascular Surgery, Lithuanian University of Health Sciences, Eivenių g. 2, Kaunas LT-50161, Lithuania.

出版信息

Dis Markers. 2020 Jun 17;2020:8521899. doi: 10.1155/2020/8521899. eCollection 2020.

DOI:10.1155/2020/8521899
PMID:32655720
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7317319/
Abstract

MATERIALS AND METHODS

Relative expression of lncRNAs CARMN, LUCAT1, SMILR, and MALAT1 was tested in clinical aortic tissue and blood plasma samples from TAA and non-TAA patients using the qRT-PCR method. The Mann-Whitney test was used to compare Ct values between the study groups. ROC curve analysis was performed to evaluate the diagnostic value of plasma lncRNAs.

RESULTS

We found significantly reduced CARMN ( = 0.033) and LUCAT1 ( = 0.009) expression in aortic tissue samples from TAA patients. Relative expression of MALAT1 ( = 0.117) and SMILR ( = 0.610) did not differ in aortic tissue between the TAA and non-TAA groups. Expression of both LUCAT1 and SMILR was significantly decreased in TAA patients' blood plasma compared to controls ( = 0.018 and = 0.032, respectively). However, only LUCAT1 showed the ability to discriminate aneurysmal disease in patients' blood plasma (AUC = 0.654, 95%CI = 0.534-0.775, = 0.018).

CONCLUSIONS

We have shown that the expression of lncRNAs CARMN and LUCAT1 is reduced in dilated aortic tissue and that the LUCAT1 and SMILR expression is lower in the blood plasma of TAA patients. Decreased LUCAT1 expression in TAA patients' blood plasma may have diagnostic potential in discriminating patients with TAA.

摘要

材料和方法

采用 qRT-PCR 法检测 TAA 和非 TAA 患者临床主动脉组织和血浆样本中 lncRNAs CARMN、LUCAT1、SMILR 和 MALAT1 的相对表达。采用 Mann-Whitney U 检验比较研究组之间的 Ct 值。采用 ROC 曲线分析评估血浆 lncRNAs 的诊断价值。

结果

我们发现 TAA 患者主动脉组织样本中 CARMN（ = 0.033）和 LUCAT1（ = 0.009）的表达明显降低。TAA 和非 TAA 组主动脉组织中 MALAT1（ = 0.117）和 SMILR（ = 0.610）的相对表达无差异。与对照组相比，TAA 患者血浆中 LUCAT1 和 SMILR 的表达均显著降低（ = 0.018 和 = 0.032）。然而，只有 LUCAT1 显示出在患者血浆中区分动脉瘤性疾病的能力（AUC = 0.654，95%CI = 0.534-0.775， = 0.018）。

结论

我们已经表明，lncRNAs CARMN 和 LUCAT1 在扩张的主动脉组织中的表达降低，并且 LUCAT1 和 SMILR 在 TAA 患者的血浆中的表达降低。TAA 患者血浆中 LUCAT1 表达降低可能具有区分 TAA 患者的诊断潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b244/7317319/dae3931ad563/DM2020-8521899.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b244/7317319/09e64693aeb1/DM2020-8521899.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b244/7317319/dae3931ad563/DM2020-8521899.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b244/7317319/09e64693aeb1/DM2020-8521899.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b244/7317319/dae3931ad563/DM2020-8521899.002.jpg

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