Bennett W A, Ellsaesser C F, Cowan B D
Department of Obstetrics and Gynecology, University of Mississippi Medical Center, Jackson 39216-4505.
Am J Reprod Immunol Microbiol. 1988 Nov;18(3):76-80. doi: 10.1111/j.1600-0897.1988.tb00239.x.
Macromolecules extracted from hydatidiform mole trophoblast inhibit mitogen-induced lymphocyte proliferation. To characterize the mechanism of this immunomodulation, we determined the effects of hydatidiform mole vesicle fluid (HMF) and tissue extracts (HME) on lymphokine function in vitro. Utilization of interleukin-1 (IL-1) and interleukin-2 (IL-2) were determined by using a lymphoma cell line (LBRM-33-1A5) and a murine T cell line (CTLL2), respectively. HMF suppressed (P less than .05) IL-2-dependent CTLL2 cell proliferation at 500 (36.4% of controls) and 50 (74.9% of controls) micrograms/ml. HME also suppressed CTLL2 proliferation (P less than .05) at 500 (46.0% of controls), 100 (67.2% of controls), 50 (71.5% of controls), and 10 (85.4% of controls) micrograms/culture ml. In contrast, HMF exhibited no effect on IL-1-stimulated LBRM-33-1A5 production of IL-2. However, 500 micrograms/ml of HME inhibited (P less than .05) IL-2 production (63.0% of controls) in the IL-1 utilization assay. This suppressive effect was probably due to a carry over of HME from the LBRM-33-1A5 culture to the target cells (CTLL2) used to measure IL-2 production. Molecular weight chromatography of an HME sample eluted an IL-2 inhibitor in a low molecular weight (35-50 kd) and high molecular weight (greater than 250 kd) fraction. These data suggest that one way in which macromolecules derived from hydatidiform mole could interfere with in vitro immunologic responses is by modulating interleukin-2 function.
从葡萄胎滋养层中提取的大分子物质可抑制有丝分裂原诱导的淋巴细胞增殖。为了阐明这种免疫调节的机制，我们在体外测定了葡萄胎囊泡液（HMF）和组织提取物（HME）对淋巴因子功能的影响。分别使用淋巴瘤细胞系（LBRM-33-1A5）和小鼠T细胞系（CTLL2）来测定白细胞介素-1（IL-1）和白细胞介素-2（IL-2）的利用情况。HMF在500（为对照组的36.4%）和50（为对照组的74.9%）微克/毫升时可抑制（P<0.05）依赖IL-2的CTLL2细胞增殖。HME在500（为对照组的46.0%）、100（为对照组的67.2%）、50（为对照组的71.5%）和10（为对照组的85.4%）微克/培养毫升时也可抑制CTLL2增殖（P<0.05）。相反，HMF对IL-1刺激的LBRM-33-1A5产生IL-2没有影响。然而，在IL-1利用试验中，500微克/毫升的HME可抑制（P<0.05）IL-2的产生（为对照组的63.0%）。这种抑制作用可能是由于HME从LBRM-33-1A5培养物中残留到用于测量IL-2产生的靶细胞（CTLL2）中。对HME样品进行分子量色谱分析，在低分子量（35-50 kd）和高分子量（>250 kd）部分洗脱出色素IL-2抑制剂。这些数据表明，来自葡萄胎的大分子物质干扰体外免疫反应的一种方式可能是通过调节白细胞介素-2的功能。
