Bennett W A, Brackin M N, Long C A, Cowan B D
Department of Obstetrics and Gynecology, University of Mississippi Medical Center, Jackson 39216-4505.
Am J Reprod Immunol. 1994 Oct;32(3):157-62. doi: 10.1111/j.1600-0897.1994.tb01107.x.
In sheep and cattle, trophoblast-derived interferons serve as signals for the maternal recognition of pregnancy and may regulate the immunologic relationship between the fetus and mother.
In this study, soluble extracts prepared from human hydatidiform mole decidua (DE) and trophoblast (HME) were screened for immunosuppressive activity using an interleukin (IL)-2-dependent T-cell line (CTLL2). Antibody neutralization studies were performed with monoclonal antibodies to alpha- and beta-interferon (IFN).
HME suppressed (P < 0.05) IL-2-stimulated (2 IU/well) CTLL2 proliferation at doses ranging from 500 (52 +/- 2% of control) to 100 (74 +/- 5%) micrograms/ml concentrations. DE also suppressed (P < or = 0.05) CTLL2 proliferation in a dose-related fashion from 500 (20 +/- 6% of control) to 100 (71 +/- 8%) micrograms/ml doses. Preincubation with the alpha- and beta-IFN antibody preparations had no effect on CTLL2 suppression by the DE sample. In contrast, the beta-IFN antibody partially neutralized the suppressive activity of HME at each of the dilutions tested. The monoclonal antibody to alpha-IFN failed to neutralize HME suppression at any of the doses tested.
These results suggest that hydatidiform mole trophoblast produces a beta-IFN-like macromolecule that may abrogate maternal rejection responses that are harmful to the developing fetal allograft.
在绵羊和牛中，滋养层来源的干扰素作为母体识别妊娠的信号，可能调节胎儿与母亲之间的免疫关系。
在本研究中，使用白细胞介素（IL）-2依赖的T细胞系（CTLL2）筛选从人葡萄胎蜕膜（DE）和滋养层（HME）制备的可溶性提取物的免疫抑制活性。用抗α和β干扰素（IFN）的单克隆抗体进行抗体中和研究。
HME在浓度范围为500（为对照的52±2%）至100（74±5%）微克/毫升时抑制（P<0.05）IL-2刺激（2国际单位/孔）的CTLL2增殖。DE也以剂量相关方式抑制（P≤0.05）CTLL2增殖，剂量范围为500（为对照的20±6%）至100（71±8%）微克/毫升。用α和β干扰素抗体制剂预孵育对DE样品抑制CTLL2没有影响。相反，β干扰素抗体在每个测试稀释度下部分中和了HME的抑制活性。α干扰素单克隆抗体在任何测试剂量下均未中和HME的抑制作用。
这些结果表明，葡萄胎滋养层产生一种β干扰素样大分子，可能消除对发育中的胎儿同种异体移植物有害的母体排斥反应。
