六型分泌系统（T6SS）在茄子上的致病性中的作用。
The involvement of the Type Six Secretion System (T6SS) in the virulence of on brinjal.
作者信息
Asolkar Trupti, Ramesh Raman
机构信息
Department of Microbiology, Goa University, Taleigao Plateau, 403 206 India.
ICAR-Central Coastal Agricultural Research Institute, Old Goa, 403 402 India.
出版信息
3 Biotech. 2020 Jul;10(7):324. doi: 10.1007/s13205-020-02311-4. Epub 2020 Jun 29.
Abstract
is an important soil-borne plant pathogen which causes bacterial wilt in a large number of crops. Bacterial Type Six Secretion System (T6SS) is known to participate in pathogenesis, bacterial interaction and inter-bacterial competition. Contribution of T6SS in the virulence of on eggplant ( L) is studied. In this study, five T6SS gene ( and ) mutants have been developed by insertional mutagenesis and the virulence of the mutants was evaluated on eggplant. In general, the T6SS mutants showed significant reduction of wilt on eggplant. mutant of gene significantly reduced the wilt from day five through day eight in petiole inoculation. In soil drench inoculation, mutant of gene reduced the wilt on eggplant and was significantly different throughout the experimental period. Other mutants, viz., and also reduced the wilt during the initial stages of disease development. This is the first report on the role of T6SS genes, and on virulence of
摘要
是一种重要的土传植物病原菌，可导致大量作物发生青枯病。已知细菌VI型分泌系统（T6SS）参与发病机制、细菌相互作用和细菌间竞争。研究了T6SS对茄子（茄属）毒力的贡献。在本研究中，通过插入诱变开发了五个T6SS基因（和）突变体，并在茄子上评估了突变体的毒力。总体而言，T6SS突变体在茄子上的萎蔫症状显著减轻。基因的突变体在叶柄接种中从第5天到第8天显著减轻了萎蔫症状。在灌根接种中，基因的突变体降低了茄子的萎蔫症状，并且在整个实验期间差异显著。其他突变体，即和在疾病发展的初始阶段也减轻了萎蔫症状。这是关于T6SS基因、和对毒力作用的首次报道
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