Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Pakistan Tobacco Board, Ministry of National Food Security and Research, Peshawar, Pakistan.
Arch Microbiol. 2023 Oct 25;205(11):358. doi: 10.1007/s00203-023-03697-4.
Volatile organic compounds (VOCs), produced by a variety of microbial species and used as biological agents, have been demonstrated to play a significant role in controlling phytopathogens. In continuation of our previous studies, we aim to elucidate the underlying mechanisms and pathways involved in interactions between pathogens and microbial VOCs. In the current study, we tested how VOCs produced by Bacillus velezensis FZB42 affect the growth of Ralstonia solanacearum TBBS1 in vitro.Query The result showed that the colony growth of R. solanacearum was reduced with an inhibition rate of 0.83 ± 0.043 as compared to the control 1.7 ± 0.076, respectively. The number of viable cells of R. solanacearum was significantly decreased to 7.68 CFU/mL as compared to the control (9.02 CFU/mL). In addition, transcriptomic analysis of R. solanacearum in response to VOCs produced by FZB42 was performed to better understand the effect of VOCs on R. solanacearum. The transcriptional response of R. solanacearum to FZB42-VOCs was determined using an Illumina RNA-seq approach. The results revealed significant changes in the expression of 2094 R. solanacearum genes, including 593 upregulated and 1501 downregulated genes. To validate the RNA-seq results, the expression of 10 genes was quantified using RT-qPCR. Furthermore, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to functionally annotate differentially expressed genes. Significant changes were observed in genes directly or indirectly related to virulence, including those related to bacterial invasion, motility, chemotaxis, and secretion systems. Overall, RNA-seq profiling provides new insights into the possible fundamental molecular mechanisms that are responsible for the reduction in growth and virulence of R. solanacearum upon application of FZB42-VOC.
挥发性有机化合物（VOCs）由多种微生物物种产生，并被用作生物制剂，已被证明在控制植物病原体方面发挥着重要作用。继我们之前的研究，我们旨在阐明病原体与微生物 VOCs 相互作用涉及的潜在机制和途径。在本研究中，我们测试了由 Bacillus velezensis FZB42 产生的 VOC 如何影响 Ralstonia solanacearum TBBS1 在体外的生长。结果表明，与对照相比，R. solanacearum 的菌落生长减少了 0.83±0.043%，分别为 1.7±0.076%。与对照相比（9.02 CFU/mL），R. solanacearum 的活菌数显著减少至 7.68 CFU/mL。此外，还对 R. solanacearum 对 FZB42 产生的 VOC 的转录组分析进行了研究，以更好地了解 VOC 对 R. solanacearum 的影响。使用 Illumina RNA-seq 方法确定了 R. solanacearum 对 FZB42-VOC 的转录响应。结果表明，R. solanacearum 的 2094 个基因的表达发生了显著变化，包括 593 个上调和 1501 个下调基因。为了验证 RNA-seq 结果，使用 RT-qPCR 定量了 10 个基因的表达。此外，还使用基因本体（GO）和京都基因与基因组百科全书（KEGG）数据库对差异表达基因进行了功能注释。观察到与毒力直接或间接相关的基因发生了显著变化，包括与细菌入侵、运动性、趋化性和分泌系统相关的基因。总的来说，RNA-seq 分析提供了新的见解，可能阐明了 FZB42-VOC 应用导致 R. solanacearum 生长和毒力降低的基本分子机制。
