Boukili Meryem, Filali Fouzia Rhazi, Lafkih Nada, Bouymajane Aziz, Sefiani Majida, Moumni Mohieddine
MD, Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Moulay Ismail University Faculty of Sciences, BP 11201 Zitoune Meknes, Morocco.
PhD, Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Moulay Ismail University Faculty of Sciences, BP 11201 Zitoune Meknes, Morocco.
Germs. 2020 Jun 2;10(2):74-80. doi: 10.18683/germs.2020.1180. eCollection 2020 Jun.
enes is one of the most pathogenic bacteria related to the consumption of contaminated food. This study aims to determine the prevalence of in raw beef meat in Meknes city of Morocco, to evaluate its pathogenicity and resistance to antimicrobials.
During four seasons, a total of 140 samples were collected from supermarkets, butcheries and Souk (weekly traditional market). The PCR method was used to examine the presence of specific and virulence genes in the isolated strains, and also to identify their serotypes. The antimicrobial resistance was determined.
The results show a prevalence of 7.14% which depends on retail sites and also on the season's variation. The majority of the strains were detected in butcheries (6 strains), and supermarkets (4 strains). Moreover, the majority of strains were detected during summer (50%). Concerning virulence genes, the seven researched genes were detected in 100% of isolated strains. The majority of strains were of the (1/2a, 1/2c, 3a and 3c) serogroup (70%), while two of them were of the (1/2b, 3b, 4b and 4d) serogroup (20%). All isolates were resistant to at least one antimicrobial, while three strains were resistant to nine tested antimicrobials. However, they were highly susceptible to amikacin, imipenem, gentamicin, sulfamethoxazole and chloramphenicol.
According to results, isolated from analyzed beef meat shows a high level of pathogenicity and resistance to the most used antimicrobials in listeriosis therapy, which calls for the severe application of quality systems at the slaughterhouses and retail sites level.
单核细胞增生李斯特菌是与食用受污染食物相关的最具致病性的细菌之一。本研究旨在确定摩洛哥梅克内斯市生牛肉中单核细胞增生李斯特菌的流行情况，评估其致病性和对抗菌药物的耐药性。
在四个季节中，共从超市、肉店和露天市场（每周传统市场）采集了140份样本。采用聚合酶链反应（PCR）方法检测分离菌株中特定基因和毒力基因的存在情况，并确定其血清型。测定抗菌药物耐药性。
结果显示流行率为7.14%，这取决于零售场所以及季节变化。大多数菌株在肉店（6株）和超市（4株）中被检测到。此外，大多数菌株在夏季被检测到（50%）。关于毒力基因，在100%的分离菌株中检测到了七个研究基因。大多数菌株属于（1/2a、1/2c、3a和3c）血清群（70%），而其中两株属于（1/2b、3b、4b和4d）血清群（20%）。所有分离株对至少一种抗菌药物耐药，而三株对九种测试抗菌药物耐药。然而，它们对阿米卡星、亚胺培南、庆大霉素、磺胺甲恶唑和氯霉素高度敏感。
根据结果，从分析的牛肉中分离出的单核细胞增生李斯特菌显示出高度的致病性以及对李斯特菌病治疗中最常用抗菌药物的耐药性，这就要求在屠宰场和零售场所层面严格应用质量体系。
