Bouymajane Aziz, Rhazi Filali Fouzia, Oulghazi Said, Ed-Dra Abdelaziz, Benhallam Faouzia, El Allaoui Abdallah, Anissi Jaouad, Sendide Khalid, Ouhmidou Bouchra, Moumni Mohieddine
MD, Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Moulay Ismail University Faculty of Sciences, BP 11201 Zitoune Meknes, Morocco.
PhD, Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Moulay Ismail University Faculty of Sciences, BP 11201 Zitoune Meknes, Morocco.
Germs. 2018 Jun 4;8(2):77-84. doi: 10.18683/germs.2018.1134. eCollection 2018 Jun.
spp. belongs to a group of pathogens which are responsible for serious infections. This study aims at highlighting the raw milk microbiological contamination and at providing data for prevalence and antimicrobial resistance of spp. isolated from raw cow's milk marketed (without any pasteurization) by street traders.
During the period of May 2015 through April 2016, 150 cow's raw milk samples were collected from street traders in Meknes city. They were examined for the identification of spp. using biochemical tests and 16S rRNA gene sequencing. The antimicrobial susceptibility of the isolates was determined.
The results showed that 11.3% (17/150) of samples were positive for the presence of spp., of which 64.7% were identified as , 17.6% as , 11.8% as and 5.9% as . The antimicrobial susceptibility showed that all spp. were resistant to ampicillin. The species , , and were resistant to streptomycin, with percentages of 52.9% (9/17), 11.8% (2/17), 11.8% (2/17), and 5.9% (1/17) respectively. All isolated strains of and were resistant to tetracycline. The multiple antibiotic resistance index was elevated in the majority of spp., reaching values higher than 0.5, indicating a risk for public health.
This study shows that the raw milk consumed by the population is contaminated with strains of resistant to antibiotics used in breeding for prophylactic purposes. This requires raising the awareness of those involved in the production and marketing of milk, so as to take measures to apply good hygienic practices and rationalize the use of zootechnical antibiotics.
[病原菌名称]属于一类可导致严重感染的病原体。本研究旨在突出生乳的微生物污染情况，并提供从街头小贩销售的（未经任何巴氏杀菌处理的）生牛乳中分离出的[病原菌名称]的流行情况及抗菌耐药性数据。
在2015年5月至2016年4月期间，从梅克内斯市的街头小贩处采集了150份生牛乳样本。通过生化试验和16S rRNA基因测序对样本进行检测以鉴定[病原菌名称]。测定分离株的抗菌药敏性。
结果显示，11.3%（17/150）的样本中存在[病原菌名称]阳性，其中64.7%被鉴定为[具体菌种1]，17.6%为[具体菌种2]，11.8%为[具体菌种3]，5.9%为[具体菌种4]。抗菌药敏性表明，所有[病原菌名称]菌株均对氨苄西林耐药。[具体菌种1]、[具体菌种2]、[具体菌种3]和[具体菌种4]对链霉素耐药，比例分别为52.9%（9/17）、11.8%（2/17）、11.8%（2/17）和5.9%（1/17）。所有分离的[具体菌种1]和[具体菌种2]菌株均对四环素耐药。大多数[病原菌名称]菌株的多重抗生素耐药指数升高，达到高于0.5的值，表明存在公共卫生风险。
本研究表明，民众消费的生乳被用于预防性养殖的抗生素耐药性[病原菌名称]菌株污染。这需要提高参与牛奶生产和销售的人员的意识，以便采取措施实施良好的卫生规范并合理使用畜牧抗生素。
