Hayward A, Laszlo M, Turman M, Vafai A, Tedder D
Department of Pediatrics, University of Colorado School of Medicine, Denver 80262.
Clin Exp Immunol. 1988 Nov;74(2):196-200.
Proliferative responses by T cells from newborn cord blood stimulated with PHA or CD3 were reduced following infection with live (but not killed) herpes simplex virus in vitro although activation (measured by calcium flux) and IL-2 production were unaffected. The impairment of proliferation was not reversed by exogenous IL-2, phosphonoacetic acid, indomethacin or anti-alpha or anti-gamma interferon antibodies. HSV DNA was detected by hybridization in DNA extracted from unseparated MNC and from subsets sorted for CD3+ and for HLA DR+ expression. HSV DNA replication was not detected by thymidine uptake and only small amounts of virus were recovered in an infectious centre assay, suggesting that infection was non-permissive. Nevertheless, in-vitro synthesis of a limited range of HSV proteins including ICP4 was detected by metabolic labelling.
体外感染活的（而非灭活的）单纯疱疹病毒后，用PHA或CD3刺激的新生儿脐带血T细胞的增殖反应降低，尽管激活（通过钙流测量）和IL-2产生未受影响。外源性IL-2、膦甲酸、吲哚美辛或抗α或抗γ干扰素抗体不能逆转增殖的损害。通过杂交在从未分离的单核细胞以及分选的CD3 +和HLA DR +表达亚群中提取的DNA中检测到HSV DNA。通过胸苷摄取未检测到HSV DNA复制，并且在感染中心试验中仅回收少量病毒，表明感染是非允许性的。然而，通过代谢标记检测到包括ICP4在内的有限范围的HSV蛋白的体外合成。
