Grogan E, Miller G, Moore T, Robinson J, Wright J
J Infect Dis. 1981 Dec;144(6):547-56. doi: 10.1093/infdis/144.6.547.
Mononuclear leukocytes freshly harvested from human umbilical cord blood were refractory to infection by herpes simplex virus (HSV) type 1, whereas cells aged in vitro for a week produced 10- to 100-fold or greater more virus. Increased sensitivity of aged cells to infection by HSV was not dependent on active DNA synthesis by the cells at the time of exposure to HSV. The aging phenomenon was not due to the outgrowth of a specific cell subpopulation that is uniquely susceptible to HSV because increased sensitivity to HSV type 1 occurred in a variety of cell subpopulations enriched or depleted of T lymphocytes, phagocytic cells, or adherent cells. About 25% of aged cells contained virions detectable by electron microscopy, considerably more cells than formed infectious centers. These virions were seen in large activated lymphocytes which are the principal cell subpopulation present in the aged cultures, but virions were also seen in small lymphocytes.
从人脐带血中新鲜采集的单核白细胞对1型单纯疱疹病毒（HSV）感染具有抗性，而在体外培养一周的细胞产生的病毒量则多出10到100倍甚至更多。老化细胞对HSV感染敏感性的增加并不依赖于细胞在接触HSV时的活跃DNA合成。老化现象并非由于对HSV具有独特易感性的特定细胞亚群的生长，因为在富含或缺乏T淋巴细胞、吞噬细胞或贴壁细胞的各种细胞亚群中，对1型HSV的敏感性均增加。约25%的老化细胞含有通过电子显微镜可检测到的病毒粒子，比形成感染中心的细胞多得多。这些病毒粒子在老化培养物中主要的细胞亚群——大型活化淋巴细胞中可见，但在小型淋巴细胞中也可见到。
