Cullen B R
Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710.
DNA. 1988 Nov;7(9):645-50. doi: 10.1089/dna.1988.7.645.
A cloned interleukin-2 (IL-2) cDNA was poorly expressed in transfected eukaryotic cells. This low expression was only partly relieved by removal of the homopolymer tail present in the 5' leader region of the encoded IL-2 mRNA. However, replacement of the natural IL-2 mRNA 5' noncoding region with a leader element derived from the efficiently translated rat preproinsulin II mRNA resulted in an mRNA molecule that was utilized effectively by the transfected cell. The enhanced expression of this chimeric IL-2 mRNA did not appear to be due to changes in the sequence near the translation initiation codon. These results suggest that the leader elements of efficiently translated mRNAs may be able to confer a higher translational efficiency on heterologous protein coding regions when present in cis.
克隆的白细胞介素-2（IL-2）cDNA在转染的真核细胞中表达不佳。这种低表达仅通过去除编码的IL-2 mRNA 5'前导区中存在的同聚物尾而部分缓解。然而，用源自高效翻译的大鼠前胰岛素原II mRNA的前导元件替换天然IL-2 mRNA 5'非编码区，产生了一种被转染细胞有效利用的mRNA分子。这种嵌合IL-2 mRNA的增强表达似乎不是由于翻译起始密码子附近序列的变化。这些结果表明，高效翻译的mRNA的前导元件在顺式存在时可能能够赋予异源蛋白质编码区更高的翻译效率。
