Iida Y, Masuda T
Department of Chemistry, Graduate School of Science, Hokkaido University, Sapporo, Japan.
Nucleic Acids Res. 1996 Sep 1;24(17):3313-6. doi: 10.1093/nar/24.17.3313.
Concerning the translation initiation signals in vertebrate mRNAs, both the ATG initiation codon and the sequences flanking the initiation codon are required to direct the position of initiation. A consensus sequence for the signal, (GCC)GCC(A or G)CCATGG, has been proposed, but actual initiation sequences differ from it to a greater or lesser degree. In the present report, the translation initiation signal sequences of rat preproinsulin and its mutant mRNAs were analyzed using a quantification method proposed previously. In this method, each 16 nt sequence in the mRNA was characterized by its sample score, which shows strength of the signal. So far, Kozak has constructed a number of preproinsulin mutant mRNAs in which nucleotides flanking the ATG codon are systematically varied, and measured the translation initiation efficiency in terms of the proinsulin product. Her experimental results were well understood on the basis of the strength of the translation initiation signal sequence.
关于脊椎动物mRNA中的翻译起始信号，ATG起始密码子及其侧翼序列对于指导起始位置都是必需的。已经提出了该信号的共有序列(GCC)GCC(A或G)CCATGG，但实际的起始序列与它或多或少存在差异。在本报告中，使用先前提出的定量方法分析了大鼠胰岛素原前体及其突变体mRNA的翻译起始信号序列。在该方法中，mRNA中的每个16个核苷酸的序列都由其样本得分来表征，该得分显示了信号强度。到目前为止，科扎克构建了许多胰岛素原前体突变体mRNA，其中ATG密码子侧翼的核苷酸被系统地改变，并根据胰岛素原产物测量了翻译起始效率。基于翻译起始信号序列的强度，她的实验结果得到了很好的理解。
