Lida Y, Masuda T
Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, Japan.
Nucleic Acids Symp Ser. 1995(34):103-4.
The signal for directing the position of translation initiation in vertebrate mRNAs requires not only ATG initiation codon but also sequences flanking the initiation codon. A consensus sequence, (GCC) GCC (A or G) CCATGG has been proposed for the signal, but actual initiation sequences differ from it in a greater or lesser degree. In the present report, the translation initiation signal sequences of rat preproinsulin mRNA and its mutant mRNAs were analyzed using a quantification method, which demonstrates that strength of the signal plays important role in determining efficiency of initiation reaction.
在脊椎动物mRNA中，指导翻译起始位置的信号不仅需要ATG起始密码子，还需要起始密码子侧翼的序列。有人提出了一种共有序列(GCC) GCC (A或G) CCATGG作为该信号，但实际的起始序列在一定程度上与它有所不同。在本报告中，使用一种定量方法分析了大鼠胰岛素原mRNA及其突变体mRNA的翻译起始信号序列，结果表明该信号的强度在决定起始反应效率方面起着重要作用。
