氯化汞与人外周血T淋巴细胞中核蛋白的结合
Binding of mercuric chloride to nuclear proteins in human peripheral blood T lymphocytes.
作者信息
Holst M, Nordlind K
机构信息
Department of Medical Cell Biology, Karolinska Institutet, Stockholm, Sweden.
出版信息
Int Arch Allergy Appl Immunol. 1988;87(4):381-3. doi: 10.1159/000234705.
The binding of labelled (203Hg) mercuric chloride to nuclear proteins of human peripheral blood T lymphocytes was studied by using polyacrylamide gel electrophoresis and autoradiography. Most of the nuclear proteins were found to be able to bind mercuric chloride; however, 4 of the proteins were heavily labelled. Of these 4 proteins, 2 migrated in the histone region, 1 comigrating exactly with histone H3. The other 2 proteins were non-histone proteins with molecular weight of approximately 25 and 43 kD.
通过聚丙烯酰胺凝胶电泳和放射自显影技术，研究了标记的（203Hg）氯化汞与人外周血T淋巴细胞核蛋白的结合情况。发现大多数核蛋白都能与氯化汞结合；然而，有4种蛋白被大量标记。在这4种蛋白中，2种在组蛋白区域迁移，其中1种与组蛋白H3完全共迁移。另外2种蛋白是非组蛋白，分子量约为25和43 kD。
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