Botany and Microbiology Department, Faculty of Science, Al-Azhar University, 11884 Nasr City, Cairo, Egypt.
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, 11884 Nasr City, Cairo, Egypt.
Int J Biol Macromol. 2020 Nov 15;163:1261-1275. doi: 10.1016/j.ijbiomac.2020.07.066. Epub 2020 Jul 10.
Bean yellow mosaic virus (BYMV) is the main cause of the mosaic and malformation of many plants, worldwide. Thus, the triggering of plant systemic resistance against BYMV is of great interest. In this endeavor, we aimed to explore the capacity of new carboxymethyl chitosan-titania nanobiocomposites (NBCs, NBC1,2) to trigger faba bean plants resistance against BYMV. Effects of NBCs on faba bean (Vicia faba L.) disease severity (DS), growth parameters, and antioxidant defense system activity were investigated under BYMV stress. Noticeably that the DS in NBCs-treated faba bean was significantly reduced compared to untreated plants. Moreover, treatment with NBCs was remarkably increased growth indices, photosynthetic pigments, membrane stability index, and relative water content compared to challenge control. Additionally, enzymatic and non-enzymatic antioxidants and total soluble protein were significantly increased. Contrary, electrolyte leakage, hydrogen peroxide, and lipid peroxidation were reduced. Interestingly that NBC1 has higher efficacy than NBC2 in triggering plant immune-system against BYMV as indicated from DS percentage (DS = 10.66% and 19.33% in case of plants treated with NBC1 and NBC2, respectively). This could be attributed to the higher content of TNPs in NBC1 (21.58%) as compared to NBC2 (14.32%). Overall, NBCs offer safe and economic antiviral agents against BYMV.
菜豆黄花叶病毒（BYMV）是引起全球许多植物花叶和畸形的主要原因。因此，触发植物对 BYMV 的系统抗性具有重要意义。在这项研究中，我们旨在探索新型羧甲基壳聚糖-二氧化钛纳米生物复合材料（NBCs，NBC1、2）引发蚕豆植物对 BYMV 抗性的能力。在 BYMV 胁迫下，研究了 NBCs 对蚕豆（Vicia faba L.）病害严重度（DS）、生长参数和抗氧化防御系统活性的影响。值得注意的是，与未处理的植物相比，NBCs 处理的蚕豆的 DS 显著降低。此外，与挑战对照相比，NBCs 处理显著增加了生长指数、光合色素、膜稳定性指数和相对含水量。此外，酶和非酶抗氧化剂以及总可溶性蛋白显著增加。相反，电解质泄漏、过氧化氢和脂质过氧化作用减少。有趣的是，NBC1 在触发植物免疫系统对抗 BYMV 方面比 NBC2 更有效，这从 DS 百分比（分别用 NBC1 和 NBC2 处理的植物的 DS 为 10.66%和 19.33%）中可以看出。这可能归因于 NBC1 中的 TNPs 含量较高（21.58%），而 NBC2 中的 TNPs 含量较低（14.32%）。总的来说，NBCs 为防治 BYMV 提供了安全、经济的抗病毒剂。
