Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan.
Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, 60 Fenwood Road, Boston, MA, 02115, USA.
Biochimie. 2020 Nov;178:158-169. doi: 10.1016/j.biochi.2020.07.002. Epub 2020 Jul 10.
Uncommon lipids in biotechnologically important Corynebacterium glutamicum and pathogen Corynebacterium striatum in genus Corynebacterium are isolated and identified by linear ion-trap multiple stage mass spectrometry (LIT MS) with high resolution mass measurement. We redefined several lipid structures that were previously mis-assigned or not defined, including cytidine diphosphate diacylglycerol (CDP-DAG), glucuronosyl diacylglycerol (GlcA-DAG), (α-d-mannopyranosyl)-(1 → 4)-(α-D-glucuronyl diacyglycerol (Man-GlcA-DAG), 1-mycolyl-2-acyl-phosphatidylglycerol (MA-PG), acyl trehalose monomycolate (acyl-TMM). We also report the structures of mycolic acid, phosphatidylglycerol, phosphatidylinositol, cardiolipin, trehalose dimycolate lipids in which many isomeric structures are present. The LIT MS approaches afford identification of the functional group, the fatty acid substituents and their regiospecificity in the molecules, revealing the biodiversities of the lipid species in two Corynebacterium strains that have played very different and important roles in human nutrition and health.
通过线性离子阱多级质谱 (LIT MS) 进行高分辨质量测量，从生物技术上重要的谷氨酸棒杆菌和棒状杆菌属病原体 Corynebacterium striatum 中分离和鉴定了不常见的脂质。我们重新定义了几个以前被错误分配或未定义的脂质结构，包括胞苷二磷酸二酰基甘油 (CDP-DAG)、葡糖醛酸二酰基甘油 (GlcA-DAG)、(α-d- 甘露吡喃糖基)-(1→4)-(α-D-葡糖醛酸二酰基甘油 (Man-GlcA-DAG)、1- -mycolyl-2-酰基-磷脂酰甘油 (MA-PG)、酰基海藻糖单酯 (acyl-TMM)。我们还报告了存在许多异构结构的类脂酸、磷脂酰甘油、磷脂酰肌醇、心磷脂、海藻糖二酯脂质的结构。LIT MS 方法能够鉴定功能基团、分子中的脂肪酸取代基及其区域特异性，揭示了在人类营养和健康中发挥非常不同和重要作用的两种棒状杆菌菌株中脂质种类的生物多样性。
