Laboratory of RNA Viruses, Department of Virus Research, Institute for Frontier Life and Medical Sciences (inFront), Kyoto University, Kyoto, Japan; Keihanshin Consortium for Fostering the Next Generation of Global Leaders in Research, Kyoto University, Kyoto, Japan.
Laboratory of RNA Viruses, Department of Virus Research, Institute for Frontier Life and Medical Sciences (inFront), Kyoto University, Kyoto, Japan; Department of Mammalian Regulatory Network, Graduate School of Biostudies, Kyoto University, Kyoto, Japan; Department of Molecular Virology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Curr Opin Virol. 2020 Oct;44:42-48. doi: 10.1016/j.coviro.2020.05.011. Epub 2020 Jul 10.
The plasmid-based reverse genetics system, which involves generation of recombinant viruses from cloned cDNA, has accelerated the understanding of clinical and virological aspects of different viruses. Borna disease virus (BoDV) is a nonsegmented, negative-strand RNA virus that causes persistent intranuclear infection in various vertebrate species. Since its first report, reverse genetics approaches with modified strategies have greatly improved rescue efficiency of recombinant BoDV and enhanced the understanding of function of each viral protein and mechanism of intranuclear persistency. Here, we summarize different reverse genetics approaches of BoDV and recent developments in the use of reverse genetics for generation of viral vectors for gene therapy and virus-like particles for potential preventive vaccines.
基于质粒的反向遗传学系统，通过从克隆 cDNA 中生成重组病毒，加速了对不同病毒的临床和病毒学方面的理解。博尔纳病病毒（BoDV）是一种非节段、负链 RNA 病毒，可导致各种脊椎动物种属的持续性核内感染。自首次报道以来，经过改良策略的反向遗传学方法极大地提高了重组 BoDV 的拯救效率，并增强了对每个病毒蛋白的功能和核内持续性机制的理解。在这里，我们总结了 BoDV 的不同反向遗传学方法，以及最近在利用反向遗传学生成基因治疗用病毒载体和潜在预防性疫苗用病毒样颗粒方面的进展。
