Chen Quansheng, Sheng Ren, Wang Pingyue, Ouyang Qin, Wang Ancheng, Ali Shujat, Zareef Muhammad, Hassan Md Mehedi
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Nov 5;241:118654. doi: 10.1016/j.saa.2020.118654. Epub 2020 Jun 26.
Malathion is an organophosphorus pesticide which could remain in agricultural products and exert irreversible harmful effects on human health. Hence, strict monitoring of malathion contents is very significant. Here, a highly sensitive fluorescent aptasensor was developed for the determination of malathion, the system was based on a cationic polymer-mediated fluorescence 'turn-off'. In this system, malathion-specific aptamers were bound to cationic polymer through electrostatic interactions. To produce fluorescence resonance energy transfer (FRET), negatively charged upconversion fluorescent nanoparticles (UCNPs) and cationic-polymer encapsulated gold nanoparticles (GNPs) were combined. This combination resulted in fluorescence quenching, and the degree of quenching was correlated with the concentration of malathion. Under optimum conditions, the fluorescence intensities were observed to decrease linearly with the rising concentration of the malathion from 0.01 to 1 μM with a detection limit of 1.42 nM. Furthermore, the developed sensor possessed good selective recognition ability for malathion and was successfully used to detect malathion in adulterated tap water and matcha samples with high accuracy.
马拉硫磷是一种有机磷农药，它可能残留在农产品中，并对人体健康产生不可逆转的有害影响。因此，严格监测马拉硫磷含量非常重要。在此，开发了一种用于测定马拉硫磷的高灵敏度荧光适配体传感器，该系统基于阳离子聚合物介导的荧光“猝灭”。在该系统中，马拉硫磷特异性适配体通过静电相互作用与阳离子聚合物结合。为了产生荧光共振能量转移（FRET），将带负电荷的上转换荧光纳米颗粒（UCNPs）和阳离子聚合物包裹的金纳米颗粒（GNPs）结合在一起。这种结合导致荧光猝灭，猝灭程度与马拉硫磷浓度相关。在最佳条件下，观察到荧光强度随马拉硫磷浓度从0.01增加到1μM呈线性下降，检测限为1.42 nM。此外，所开发的传感器对马拉硫磷具有良好的选择性识别能力，并成功用于高精度检测掺假自来水和抹茶样品中的马拉硫磷。
