Yiwei Xu, Xupeng Jia, Sennan Yang, Mengrui Cao, Baoshan He, Wenjie Ren, Zhiguang Suo
National Engineering Laboratory/Key Laboratory of Henan province, School of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China.
Henan Institute of Food and Salt Industry Inspection Technology, Zhengzhou 450003, China.
Food Chem X. 2024 Sep 17;24:101835. doi: 10.1016/j.fochx.2024.101835. eCollection 2024 Dec 30.
An aptasensor with dual-outputs was developed for malathion detection. Fe-MOF was synthesized to design favorable signal probes for catalytic amplification. Owing to the excellent peroxidase-like activity of Fe-MOF, the redox reaction was catalyzed to produce the dual-outputs of colorimetric and electrochemical. In this sensing strategy, malathion was captured by the aptamer on sensing interface, leading to the release of signal probe. Thanks to the catalytic amplification of Fe-MOF and the high capture effect of aptamer, the aptasensor produced a sensitive response for malathion. Based on the dual-signals of absorbance and current, the detection method for malathion was developed ranging from 10 ng/mL to 500 ng/mL. The detection limit of malathion was 5.8 ng/mL for colorimetric output and 4.6 ng/mL for electrochemical output. Furthermore, the aptasensor exhibited high specificity and good repeatability in malathion detection. Finally, the aptasensor was applied to detect malathion in fruit and vegetable samples with satisfactory recovery.
开发了一种用于检测马拉硫磷的具有双输出的适配体传感器。合成了铁基金属有机框架（Fe-MOF）以设计用于催化放大的良好信号探针。由于Fe-MOF具有优异的类过氧化物酶活性，催化氧化还原反应产生比色和电化学双输出。在这种传感策略中，马拉硫磷被传感界面上的适配体捕获，导致信号探针释放。得益于Fe-MOF的催化放大作用和适配体的高捕获效果，该适配体传感器对马拉硫磷产生了灵敏响应。基于吸光度和电流的双信号，开发了10 ng/mL至500 ng/mL范围内的马拉硫磷检测方法。马拉硫磷的比色输出检测限为5.8 ng/mL，电化学输出检测限为4.6 ng/mL。此外，该适配体传感器在马拉硫磷检测中表现出高特异性和良好的重复性。最后，该适配体传感器应用于检测水果和蔬菜样品中的马拉硫磷，回收率令人满意。
