State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China.
State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Genes (Basel). 2020 Jul 9;11(7):772. doi: 10.3390/genes11070772.
The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological function mode in plants. Based on the results of the current investigation, the gene crucially affects filament elongation and anther dehiscence of the stamen in cotton. Both excessive and lower expression of the gene lead to defective stamen development, resulting in shortened filaments and indehiscent anthers with pollen abortion. The results also showed that the phenotype of the shortened filaments was negatively correlated with anther defects in the seesaw model under the ectopic expression of . Moreover, our results notably indicated that the gene requires accurate expression and exhibits a sensitive dose effect for its proper function. This report has important fundamental and practical significance in crop science, and has crucial prospects for genetic engineering of new cytoplasmic male sterility lines and breeding of crop hybrid varieties.
同源亮氨酸拉链/EF 手包含跨膜（LETMs）在广泛的真核生物中高度保守。在动物中已经确定了涉及生物过程的 LETM 功能特征，但对植物中 LETM 生物功能模式知之甚少。基于当前研究的结果，该基因在棉花中对花丝的伸长和花粉囊开裂至关重要。基因的过度和较低表达都会导致雄蕊发育缺陷，导致花丝缩短和花粉败育的花粉囊开裂。结果还表明，在异位表达的情况下，短花丝的表型与摆动模型中的花粉囊缺陷呈负相关。此外，我们的结果还表明，该基因需要准确表达，并表现出对其正常功能的敏感剂量效应。本报告在作物科学中具有重要的基础和实践意义，对新型细胞质雄性不育系的遗传工程和作物杂交品种的培育具有重要的前景。
