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鼓室内注射后点击交联水凝胶贮库的体内成像

In Vivo Imaging of Click-Crosslinked Hydrogel Depots Following Intratympanic Injection.

作者信息

Ju Hyeon Jin, Park Mina, Park Ji Hoon, Shin Gi Ru, Choi Hak Soo, Suh Myung-Whan, Kim Moon Suk

机构信息

Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea.

Department of Otorhinolaryngology-Head and Neck Surgery, Seoul Medical Center, Seoul 05505, Korea.

出版信息

Materials (Basel). 2020 Jul 9;13(14):3070. doi: 10.3390/ma13143070.

DOI:10.3390/ma13143070
PMID:32660032
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7412526/
Abstract

In this study, we developed injectable intratympanic hyaluronic acid (HA) depots for the treatment of hearing loss. We prepared an injectable click-crosslinking formulation by modifying HA with tetrazine (HA-TET) and trans-cyclooctene (HA-TCO), which crosslinked to form an HA depot (Cx-HA). Preparation of the click-crosslinking HA formulation was facile, and Cx-HA depot formation was reproducible. Additionally, the Cx-HA hydrogel was significantly stiffer than HA hydrogel. To monitor the degradation pattern of hydrogels, we mixed a zwitterionic near-infrared (NIR) fluorophore (e.g., ZW800-1C) in the click-crosslinking HA formulation. Then, HA-TET and HA-TCO solutions containing ZW800-1C were loaded separately into the compartments of a dual-barrel syringe for intratympanic injection. The Cx-HA depots formed quickly, and an extended residence time in the tympanic cavity was confirmed by performing NIR fluorescence imaging. We have successfully prepared an injectable click-crosslinking HA formulation that has promise as an intratympanic drug depot.

摘要

在本研究中，我们开发了用于治疗听力损失的可注射鼓室内透明质酸（HA）贮库。我们通过用四嗪（HA-TET）和反式环辛烯（HA-TCO）修饰HA制备了一种可注射的点击交联制剂，它们交联形成HA贮库（Cx-HA）。点击交联HA制剂的制备很简便，且Cx-HA贮库的形成具有可重复性。此外，Cx-HA水凝胶比HA水凝胶明显更硬。为了监测水凝胶的降解模式，我们在点击交联HA制剂中混合了一种两性离子近红外（NIR）荧光团（例如ZW800-1C）。然后，将含有ZW800-1C的HA-TET和HA-TCO溶液分别装入双筒注射器的隔室中用于鼓室内注射。Cx-HA贮库迅速形成，通过进行NIR荧光成像证实其在鼓室内的停留时间延长。我们成功制备了一种有前景作为鼓室内药物贮库的可注射点击交联HA制剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/34846c3fc8f7/materials-13-03070-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/320edac53e0e/materials-13-03070-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/8565558d8309/materials-13-03070-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/1e0a46b4ba23/materials-13-03070-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/7707ca0660c9/materials-13-03070-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/34846c3fc8f7/materials-13-03070-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/320edac53e0e/materials-13-03070-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/8565558d8309/materials-13-03070-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/1e0a46b4ba23/materials-13-03070-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/7707ca0660c9/materials-13-03070-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c3/7412526/34846c3fc8f7/materials-13-03070-g005.jpg

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