Electrical and Computer Engineering, Lehigh University, Bethlehem, PA 18015, USA.
Electrical and Computer Engineering, Cornell University, New York, NY 14850, USA.
Sensors (Basel). 2020 Jul 9;20(14):3844. doi: 10.3390/s20143844.
Single-connection in situ calibration using biocompatible solutions is demonstrated in single-cell sensing from 0.5 to 9 GHz. The sensing is based on quickly trapping and releasing a live cell by dielectrophoresis on a coplanar transmission line with a little protrusion in one of its ground electrodes. The same transmission line is used as the calibration standard when covered by various solutions of known permittivities. The results show that the calibration technique may be precise enough to differentiate cells of different nucleus sizes, despite the measured difference being less than 0.01 dB in the deembedded scattering parameters. With better accuracy and throughput, the calibration technique may allow broadband electrical sensing of live cells in a high-throughput cytometer.
本文展示了一种在 0.5 至 9GHz 范围内用于单细胞感应的单连接原位校准方法，该方法使用生物相容性溶液。该感应方法基于在共面传输线的一个地电极上的小突起处通过介电泳快速捕获和释放活细胞，当该传输线被具有已知介电常数的各种溶液覆盖时，该传输线被用作校准标准。结果表明，尽管在去嵌入散射参数中测量的差异小于 0.01dB，但校准技术可能足够精确，可以区分不同细胞核大小的细胞。该校准技术具有更好的准确性和吞吐量，可实现在高通量细胞仪中对活细胞的宽带电感应。
