Department of Comparative Biomedicine and Food Science (BCA), University of Padova, Viale dell'Università 16, I-35020 Legnaro, Padova, Italy.
Institute of Animal Science and Technology, Group of Aquaculture and Biodiversity, Universitat Politècnica de València, Camino de Vera, 14, 46071 València, Spain.
Int J Mol Sci. 2020 Jul 9;21(14):4871. doi: 10.3390/ijms21144871.
The present study evaluated the effects of wash-out on the fatty acid (FA) composition in the muscles of Mediterranean yellowtail. After 109 days during which fish were fed either a fish oil (FO)-based diet (FO 100) or a diet (FO 0) in which FO was completely substituted by vegetable oils, all fish were subjected to a wash-out with FO 100 diet for 90 days. The FA profile of muscles in fish fed FO 0 diet at the beginning of the experiment reflected that of dietary vegetable oils, rich in linoleic acid (LA), and α-linolenic acid (ALA), and was deficient in AA (arachidonic acid), EPA (eicosapentaenoic acid), and DHA (docosahexaenoic acid). No essential FA were fully restored in fish previously fed FO 0 diet on 45th or 90th day of wash-out. At the end of wash-out, the FA composition showed that AA, EPA, and DHA in the white muscles increased by +33%, +16%, and +43% ( < 0.001), respectively. Similarly, AA and DHA in the red muscles increased by +33% and +41% respectively, while EPA remained similar to fish fed FO 0 diet exclusively. Therefore, a 90-d wash-out can partially improve the FA profile in muscles of Mediterranean yellowtail previously fed vegetable oil-based diets.
本研究评估了冲洗对地中海黄尾鱼肌肉中脂肪酸 (FA) 组成的影响。在鱼连续 109 天喂食鱼油 (FO) 基础饲料 (FO 100) 或完全用植物油替代 FO 的饲料 (FO 0) 之后，所有鱼都用 FO 100 饲料进行了 90 天的冲洗。在实验开始时喂食 FO 0 饲料的鱼的肌肉 FA 谱反映了饲料中植物油的特征，富含亚油酸 (LA) 和α-亚麻酸 (ALA)，而 AA（花生四烯酸）、EPA（二十碳五烯酸）和 DHA（二十二碳六烯酸）则不足。在冲洗的第 45 天或第 90 天，之前喂食 FO 0 饲料的鱼没有完全恢复任何必需的 FA。在冲洗结束时，肌肉的 FA 组成表明，白色肌肉中的 AA、EPA 和 DHA 分别增加了 +33%、+16%和 +43%（<0.001）。同样，红色肌肉中的 AA 和 DHA 分别增加了 +33%和 +41%，而 EPA 与仅喂食 FO 0 饲料的鱼相似。因此，90 天的冲洗可以部分改善之前喂食植物油饲料的地中海黄尾鱼肌肉中的 FA 谱。
