Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Animal Virome and Diagnostic Development Research Group, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
BMC Vet Res. 2020 Jul 13;16(1):240. doi: 10.1186/s12917-020-02467-4.
Feline morbillivirus (FeMV) has been discovered in domestic cats associated with tubulointerstitial nephritis, but FeMV is also detected in healthy cats. This research aimed to identify and characterize the FeMV strains detected in a Thai cat population.
Two-hundred and ninety-two samples (131 urine and 161 blood) derived from 261 cats (61 sheltered and 200 household cats) were included for investigating the FeMV prevalence using real-time reverse transcription PCR. The overall prevalence of FeMV detection was 11.9% (31/261) among both samples, which accounted for 14.5% (19/131) and 7.5% (12/161) of the urine and blood samples, respectively. Among the FeMV-PCR positive cats, the FeMV-detected prevalence was insignificantly associated with healthy cats (58.1%; 18/31) or urologic cats (41.9%; 13/31). Full-length genome analysis of these FeMV-Thai strains revealed that their genomes clustered together in the FeMV-1A clade with up to 98.5% nucleotide identity. Selective pressure analysis showed that overall FeMV-1 has undergone negative selection, while positive selection sites were more frequently observed in the phosphoprotein gene.
The detected FeMV infections in the Thai cat population were not correlated with urologic disorders, although the virus was more detectable in urine samples. The genetic patterns among the FeMV-1 Thai strains were more consistent. A large-scale study of FeMV in Thai cat samples is needed for further elucidation.
猫传染性涎瘤病毒（FeMV）已在与肾小管间质性肾炎相关的家猫中被发现，但也在健康猫中检测到 FeMV。本研究旨在鉴定和描述在泰国猫群中检测到的 FeMV 株。
使用实时逆转录 PCR 对来自 261 只猫（61 只收容猫和 200 只家猫）的 292 份样本（131 份尿液和 161 份血液）进行了 FeMV 流行率调查。在这两种样本中，FeMV 的总检出率为 11.9%（31/261），分别占尿液样本的 14.5%（19/131）和血液样本的 7.5%（12/161）。在 FeMV-PCR 阳性的猫中，FeMV 检出率与健康猫（58.1%；18/31）或泌尿猫（41.9%；13/31）之间无显著相关性。对这些 FeMV-泰国株的全长基因组分析表明，它们的基因组聚集在 FeMV-1A 分支中，核苷酸同一性高达 98.5%。选择压力分析显示，总体上 FeMV-1 经历了负选择，而在磷蛋白基因中观察到更多的正选择位点。
在泰国猫群中检测到的 FeMV 感染与泌尿系统疾病无关，尽管该病毒在尿液样本中更易检测到。FeMV-1 泰国株之间的遗传模式更为一致。需要对泰国猫样本中的 FeMV 进行大规模研究，以进一步阐明。
