Ledoux Jean-Baptiste, Cruz Fernando, Gómez-Garrido Jèssica, Antoni Regina, Blanc Julie, Gómez-Gras Daniel, Kipson Silvija, López-Sendino Paula, Antunes Agostinho, Linares Cristina, Gut Marta, Alioto Tyler, Garrabou Joaquim
CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Porto, 4050-123, Portugal
Institut de Ciències del Mar, CSIC, Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain.
G3 (Bethesda). 2020 Sep 2;10(9):2941-2952. doi: 10.1534/g3.120.401371.
The octocoral, , is a habitat-forming anthozoan with a key ecological role in rocky benthic and biodiversity-rich communities in the Mediterranean and Eastern Atlantic. Shallow populations of in the North-Western Mediterranean are severely affected by warming-induced mass mortality events (MMEs). These MMEs have differentially impacted individuals and populations of (, varied levels of tissue necrosis and mortality rates) over thousands of kilometers of coastal areas. The eco-evolutionary processes, including genetic factors, contributing to these differential responses remain to be characterized. Here, we sequenced a individual with short and long read technologies, producing 169.98 Gb of Illumina paired-end and 3.55 Gb of Oxford Nanopore Technologies (ONT) reads. We obtained a genome assembly accounting for 607 Mb in 64,145 scaffolds. The contig and scaffold N50s are 19.15 Kb and 23.92 Kb, respectively. Despite of the low contiguity of the assembly, its gene completeness is relatively high, including 75.8% complete and 9.4% fragmented genes out of the 978 metazoan genes contained in the metazoa_odb9 database. A total of 62,652 protein-coding genes have been annotated. This assembly is one of the few octocoral genomes currently available. This is undoubtedly a valuable resource for characterizing the genetic bases of the differential responses to thermal stress and for the identification of thermo-resistant individuals and populations. Overall, having the genome of will facilitate studies of various aspects of its evolutionary ecology and elaboration of effective conservation plans such as active restoration to overcome the threats of global change.
八放珊瑚是一种形成栖息地的珊瑚虫，在地中海和东大西洋的岩石底栖生物和生物多样性丰富的群落中具有关键的生态作用。地中海西北部浅海的八放珊瑚种群受到变暖导致的大规模死亡事件（MMEs）的严重影响。这些MMEs在数千公里的沿海区域对八放珊瑚的个体和种群产生了不同程度的影响（例如，组织坏死和死亡率水平各异）。导致这些不同反应的生态进化过程，包括遗传因素，仍有待确定。在这里，我们使用短读长和长读长技术对一个八放珊瑚个体进行了测序，产生了169.98 Gb的Illumina双端读数和3.55 Gb的牛津纳米孔技术（ONT）读数。我们获得了一个八放珊瑚基因组组装，由64,145个支架组成，总计607 Mb。重叠群和支架的N50分别为19.15 Kb和23.92 Kb。尽管组装的连续性较低，但其基因完整性相对较高，在metazoa_odb9数据库中包含的978个后生动物基因中，完整基因占75.8%，片段化基因占9.4%。总共注释了62,652个蛋白质编码基因。这个组装是目前可用的少数八放珊瑚基因组之一。这无疑是一个宝贵的资源，可用于确定对热应激不同反应的遗传基础，以及识别耐热个体和种群。总体而言，拥有八放珊瑚的基因组将有助于研究其进化生态学的各个方面，并制定有效的保护计划，如积极恢复，以应对全球变化的威胁。
