Department of Pediatrics, 7284University of Washington, Seattle, WA, USA.
Department of Surgery, 7284University of Washington, Seattle, WA, USA.
Appl Spectrosc. 2020 Oct;74(10):1263-1273. doi: 10.1177/0003702820938867. Epub 2020 Jul 14.
In critically ill patients, direct knowledge of intracellular pO would allow for identification of cellular hypoxia, which when prolonged leads to organ failure. We have developed a visible-near-infrared optical system that noninvasively measures myoglobin saturation, which is directly related to intracellular pO, from the surface of the skin. We used an animal model of graded hypoxia from low levels of inspired oxygen ( = 5) and verified that low intracellular pO is correlated with high steady-state serum lactate values. In addition, the pO gradient between arterial blood and inside muscle cells was 83 mm Hg at 21% O, but fell to 24 mm Hg at 8% O. Continuous myoglobin saturation measurement in skeletal muscle displayed the same trends as cerebral oxygenation with no lag in changes over time, demonstrating its relevance as a measure of systemic oxygenation.
在危重病患者中，直接了解细胞内的 pO2 可以识别细胞缺氧，而长时间的细胞缺氧会导致器官衰竭。我们开发了一种可见-近红外光学系统，可以从皮肤表面无创地测量与细胞内 pO2 直接相关的肌红蛋白饱和度。我们使用了一个从低水平吸入氧（ = 5）逐渐诱导缺氧的动物模型，并验证了低细胞内 pO2 与高稳态血清乳酸值相关。此外，在 21%的 O2 时，动脉血和肌细胞内的 pO2 梯度为 83 mm Hg，但在 8%的 O2 时降至 24 mm Hg。骨骼肌的连续肌红蛋白饱和度测量与脑氧合显示出相同的趋势，没有时间上的滞后变化，表明它作为全身氧合测量的相关性。
