Biomedical Research Center, Cardiovascular Research Lab, Department of Cardiac and Thoracic Vascular Surgery, University Medical Center, Philipps-University of Marburg/Lahn, Marburg/Lahn, Germany.
J Surg Res. 2012 Jul;176(1):337-42. doi: 10.1016/j.jss.2011.04.064. Epub 2011 May 26.
In this study, the microvascular tissue oxygenation and oxidative muscle metabolism during graded hypoxia and reoxygenation were examined in a rabbit model by near-infrared (NIR) spectroscopy and correlated with high-energy phosphates measured by (31)P nuclear magnetic resonance (NMR) spectroscopy.
Graded hypoxia was performed in a New Zealand rabbit model (n = 20, 2.0 ± 0.4 kg) by a stepwise reduction of the fraction of inspired oxygen (FiO(2)) from 0.3 to 0.05 (intervention group versus control group). Recovery and reoxygenation were achieved using FiO(2) of 0.3. A noninvasive NIR spectroscopy sensor and NMR probe was positioned on the surface of the prepared pedicled latissimus dorsi muscle. Microvascular tissue oxygenation (oxyhemoglobin, HbO(2); deoxyhemoglobin, HHb) and redox state of cytochrome oxidase (CytOx) were measured by NIR spectroscopy and correlated with standard values of oxidative muscle metabolism (phosphocreatine, PCr; adenosine triphosphate, ATP) measured by time-resolved (31)P NMR spectroscopy (4.7T).
Significant correlation was found between PCr and HbO(2) (r = 0.85, P < 0.001) and HHb (r = -0.75, P < 0.001). β-ATP levels correlated significantly with CytOx (r = 0.87, P < 0.001).
The data suggest that changes in high-energy phosphates (PCr- and ATP-levels) correlate closely with microvascular tissue oxygenation (HbO(2), HHb, CytOx) measured by NIR spectroscopy.
本研究通过近红外光谱（NIR）和（31）P 磁共振波谱（NMR）检测新西兰兔模型在分级低氧和再复氧过程中的微血管组织氧合和氧化肌肉代谢，并将其与高能磷酸物进行相关分析。
20 只新西兰兔（2.0±0.4kg）行逐步降低吸入氧分数（FiO2）至 0.05（干预组与对照组），以建立分级低氧模型。使用 FiO2 0.3 实现复氧。在准备好的带蒂背阔肌表面放置一个非侵入性的近红外光谱传感器和 NMR 探头。通过 NIR 光谱测量微血管组织氧合（氧合血红蛋白，HbO2；脱氧血红蛋白，HHb）和细胞色素氧化酶的氧化还原状态（CytOx），并与通过时间分辨（31）P NMR 光谱（4.7T）测量的标准氧化肌肉代谢值（磷酸肌酸，PCr；三磷酸腺苷，ATP）进行相关分析。
PCr 与 HbO2（r=0.85，P<0.001）和 HHb（r=-0.75，P<0.001）之间存在显著相关性。β-ATP 水平与 CytOx 显著相关（r=0.87，P<0.001）。
研究数据表明，高能磷酸物（PCr 和 ATP 水平）的变化与通过 NIR 光谱测量的微血管组织氧合（HbO2、HHb、CytOx）密切相关。
