Department of Pharmacology, Faculty of Pharmacy, University of Seville, Seville, Spain.
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Seville, Seville, Spain.
Int J Food Sci Nutr. 2021 Mar;72(2):184-194. doi: 10.1080/09637486.2020.1793305. Epub 2020 Jul 14.
New neuroprotective treatments of natural origin are being investigated. Both, plant extracts and isolated compounds have shown bioactive effects. Hempseed is known for its composition of fatty acids, proteins, fibre, vitamins, as well as a large number of phytochemical compounds. After a defatting process of the seeds, hydroxycinnamic acids and its amine derivatives are the majoritarian compounds in an ethyl acetate fraction (EAF). In the present study, we investigated effect on neuronal enzymes: MAO-A, MAO-B, tyrosinase and acetylcholinesterase. Besides, the effect of EAF on striatal biogenic amines in mice was evaluated. Both, EAF and isolated compounds (-caffeoyltyramine and -coumaroyltyramine), showed inhibitory action on MAO-A, MAO-B and tyrosinase. Furthermore, an increasing of biogenic amines was observed in the corpus striatum of the mice, after administration of EAF. These findings show that EAF and the hydroxycinnamic acid derivatives may represent a potential treatment in degenerative neuronal diseases.
正在研究新的天然来源的神经保护治疗方法。植物提取物和分离的化合物都表现出了生物活性。 Hempseed 以其脂肪酸、蛋白质、纤维、维生素以及大量植物化学化合物而闻名。 在种子脱脂过程之后，羟基肉桂酸及其胺衍生物是乙酸乙酯馏分 (EAF) 中的主要化合物。 在本研究中，我们研究了 EAF 对神经元酶的影响：MAO-A、MAO-B、酪氨酸酶和乙酰胆碱酯酶。 此外，还评估了 EAF 对小鼠纹状体生物胺的影响。 EAF 和分离的化合物（-咖啡酰酪胺和 -香豆酰酪胺）都对 MAO-A、MAO-B 和酪氨酸酶表现出抑制作用。 此外，在给予 EAF 后，在纹状体中观察到生物胺的增加。 这些发现表明，EAF 和羟基肉桂酸衍生物可能代表退行性神经疾病的潜在治疗方法。
