Zhang Pei, Liu Quan, Fan Zongqing, Wu Min, Zhu Kun, Zhou Jiansheng, Xiang Ping
Department of Orthopaedics, the First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui, 233000, P.R.China.
Department of Orthopaedics, the First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui, 233000, P.R.China;Key Laboratory of Tissue and Transplant in Anhui Province, the First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui, 233000, P.R.China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2020 Jul 15;34(7):848-853. doi: 10.7507/1002-1892.201912048.
To investigate the effect of vascularized peroneus longus tendon graft reconstruction on anterior cruciate ligament (ACL) insertion in rabbits.
Eighty healthy New Zealand white rabbits were harvested to prepare ACL injury models and randomly divided into two groups ( =40). The ACL was reconstructed with vascularized peroneus longus tendon graft in group A and peroneus longus tendon graft without blood supply in group B. The survival of animals and the healing of incision were observed after operation; the grafts were taken for gross and histological observations at 4, 8, and 16 weeks; the biomechanical test of the grafts was carried out to record the maximum tensile strength and incidence of ACL insertion rupture at 8 and 16 weeks.
All animals survived until the experiment completed. General observation showed that the tunnel was combined with grafts, the vascular infiltration was abundant, and no obvious boundary between the tunnel and grafts existed at 16 weeks in group A; there was still an obvious boundary between the tunnel and graft in group B. Histological observation showed that the collagen fibers between tendon and bone in group A increased gradually, the dense fiber connection was formed, and the "tidal-line" like structure similar to the normal ACL insertion was formed at 16 weeks; but the"tidal-line" like structure was not obvious in group B. Biomechanical test showed that there was no significant difference in the incidence of ACL insertion rupture at 8 and 16 weeks between group A and group B ( =0.680; =0.590), but the maximum tensile strength at 8 and 16 weeks were higher in group A than in group B ( =18.503, =0.001; =25.391, =0.001).
The vascularized peroneus longus tendon graft for ACL reconstruction can obviously promote the outcome of the ACL insertion.
探讨带血管腓骨长肌腱移植重建兔前交叉韧带（ACL）止点的效果。
选取80只健康新西兰白兔制备ACL损伤模型，随机分为两组（每组 =40只）。A组采用带血管腓骨长肌腱移植重建ACL，B组采用无血供腓骨长肌腱移植。术后观察动物存活情况及切口愈合情况；于术后4、8、16周取移植肌腱进行大体及组织学观察；于术后8、16周对移植肌腱进行生物力学测试，记录最大拉伸强度及ACL止点断裂发生率。
所有动物均存活至实验结束。大体观察显示，A组16周时隧道与移植肌腱融合，血管浸润丰富，隧道与移植肌腱间无明显界限；B组隧道与移植肌腱间仍有明显界限。组织学观察显示，A组肌腱与骨间胶原纤维逐渐增多，形成致密纤维连接，16周时形成类似正常ACL止点的“潮线”样结构；B组“潮线”样结构不明显。生物力学测试显示，A、B两组术后8、16周ACL止点断裂发生率差异无统计学意义（ =0.680； =0.590），但A组术后8、16周最大拉伸强度均高于B组（ =18.503， =0.001； =25.391， =0.001）。
带血管腓骨长肌腱移植重建ACL可明显促进ACL止点的愈合效果。
