Costa Tania Maria, Lenzi Juliana, da Silva Filho Hercílio Higino, Goulart Juliane Araújo Greinert, Alberton Michele Debiasi, Tavares Lorena Benathar Ballod, de Oliveira Débora
Department of Chemical and Food Engineering, Federal University of Santa Catarina, Trindade, Florianópolis, Brazil.
Graduate Program in Environmental Engineering, Regional University of Blumenau, Blumenau, Brazil.
Nat Prod Res. 2021 Dec;35(23):5530-5534. doi: 10.1080/14786419.2020.1793146. Epub 2020 Jul 15.
Natural antiparasitic agents are used as a strategy for advances in the treatment of neglected tropical diseases (NTDs), such as giardiasis. The present paper identified the compounds produced by mycelium grown in submerged fermentation and investigated the antigiardial activity potential of extracts . mycelium crude extract () was obtained by the maceration process and after purification in column, thirty compounds were identified by gas chromatography - mass spectrometry (GC-MS) in hexane sub-fraction (). To determine the antigiardial potential, different parts of the purified have been evaluated, where hexane () and dichloromethane () fractions showed reduction levels higher than 90% of the trophozoites at 100 μg mL. This antigiardial activity was investigated aiming to explore the possibility of mycelium extract as a novel antigiardial compound to the treatment of human giardiasis.
天然抗寄生虫剂被用作治疗被忽视的热带疾病（如贾第虫病）取得进展的一种策略。本文鉴定了在深层发酵中生长的菌丝体产生的化合物，并研究了提取物的抗贾第虫活性潜力。菌丝体粗提物通过浸渍法获得，柱纯化后，通过气相色谱 - 质谱联用（GC-MS）在己烷亚组分中鉴定出30种化合物。为了确定抗贾第虫潜力，对纯化提取物的不同部分进行了评估，其中己烷馏分和二氯甲烷馏分在100μg/mL时对贾第虫滋养体的减少水平高于90%。对这种抗贾第虫活性进行了研究，旨在探索菌丝体提取物作为一种新型抗贾第虫化合物用于治疗人类贾第虫病的可能性。
