A根提取物及其馏分具有抗贾第虫活性且不影响细胞活力。
A Root Extract and Fractions Exhibit Antigiardial Activity without Affecting Cell Viability.
作者信息
Quintal-Novelo Carlos, Valencia-Chan Lía, Chávez-González Antonieta, Rangel-Méndez Jorge, Moo-Puc Rosa
机构信息
High Specialty Medical Unit, Medical Center "Ignacio García Téllez", Mexican Institute of Social Security, Mérida, Yucatán, Mexico.
Yucatan Medical Research Unit, High Specialty Medical Unit, Medical Center "Ignacio García Téllez", Mexican Institute of Social Security, Mérida, Yucatán, Mexico.
出版信息
Iran J Parasitol. 2022 Apr-Jun;17(2):259-267. doi: 10.18502/ijpa.v17i2.9544.
BACKGROUND
The gastrointestinal parasite causes giardiasis. Its treatment with standard drugs produces side effects and improper treatment can generate resistant strains. New antigiardial compounds are needed. An analysis was done to identify the antigiardial activity of , a plant used in traditional Mayan medicine to treat stomach and bowel pain. We aimed to assess the efficacy of roots against and their effect on cells viability.
METHODS
A methanol extract was done of the root and then fractionated. The extract and fractions were tested in vitro on trophozoites and their effect on cell viability was quantified by flow cytometry. The active extract and fractions were analyzed by gas chromatography-mass spectrometry and high-performance liquid chromatography.
RESULTS
The hexane fraction exhibited potent activity against (IC = 0.08 μg/mL). Its principal component was an anthraquinone-type compound. None of the fractions were toxic to human promyelocytic leukemia, chronic myelogenous leukemia and human mononuclear cells.
CONCLUSION
The medicinal plant contains promising bioactive agents with antigiardial activity and deserves further research.
背景
这种胃肠道寄生虫会引发贾第虫病。用标准药物治疗会产生副作用，且不当治疗会产生耐药菌株。因此需要新的抗贾第虫化合物。我们进行了一项分析，以确定一种在玛雅传统医学中用于治疗肠胃疼痛的植物的抗贾第虫活性。我们旨在评估该植物根对贾第虫的疗效及其对细胞活力的影响。
方法
对该植物的根进行甲醇提取，然后进行分离。提取物和分离物在体外对贾第虫滋养体进行测试，并通过流式细胞术对其对细胞活力的影响进行定量。对活性提取物和分离物进行气相色谱 - 质谱分析和高效液相色谱分析。
结果
己烷分离物对贾第虫表现出强效活性（IC = 0.08μg/mL）。其主要成分是一种蒽醌类化合物。没有一个分离物对人早幼粒细胞白血病、慢性粒细胞白血病和人单核细胞有毒性。
结论
这种药用植物含有具有抗贾第虫活性的有前景的生物活性剂，值得进一步研究。
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