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稳定型和不稳定型心绞痛患者循环 microRNA 表达谱。

Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable Angina.

机构信息

Research Experimental Center, Meizhou People's Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou 514031, P. R. China.

Center for Cardiovascular Diseases, Meizhou People's Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou 514031, P. R. China.

出版信息

Clinics (Sao Paulo). 2020;75:e1546. doi: 10.6061/clinics/2020/e1546. Epub 2020 Jul 10.

DOI:10.6061/clinics/2020/e1546
PMID:32667489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7337223/
Abstract

OBJECTIVES

High incidence and case fatality of unstable angina (UA) is, to a large extent, a consequence of the lack of highly sensitive and specific non-invasive markers. Circulating microRNAs (miRNAs) have been widely recommended as potential biomarkers for numerous diseases. In the present study, we characterized distinctive miRNA expression profiles in patients with stable angina (SA), UA, and normal coronary arteries (NCA), and identified promising candidates for UA diagnosis.

METHODS

Serum was collected from patients with SA, UA, and NCA who visited the Department of Cardiovascular Diseases of the Meizhou People's Hospital. Small RNA sequencing was carried out on an Illumina HiSeq 2500 platform. miRNA expression in different groups of patients was profiled and then confirmed based on that in an independent set of patients. Functions of differentially expressed miRNAs were predicted using gene ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis.

RESULTS

Our results indicated that circulating miRNA expression profiles differed between SA, UA, and NCA patients. A total of 36 and 161 miRNAs were dysregulated in SA and UA patients, respectively. miRNA expression was validated by reverse transcription quantitative polymerase chain reaction.

CONCLUSION

The results suggest that circulating miRNAs are potential biomarkers of UA.

摘要

目的

不稳定型心绞痛（UA）的高发病率和病死率在很大程度上是由于缺乏高度敏感和特异的非侵入性标志物所致。循环 microRNAs（miRNAs）已被广泛推荐为多种疾病的潜在生物标志物。本研究旨在对稳定型心绞痛（SA）、UA 和正常冠状动脉（NCA）患者的特征性 miRNA 表达谱进行表征，并确定用于 UA 诊断的有前途的候选标志物。

方法

收集梅州市人民医院心血管疾病科 SA、UA 和 NCA 患者的血清。在 Illumina HiSeq 2500 平台上进行小 RNA 测序。对不同组患者的 miRNA 表达进行分析，并在独立组患者中进行验证。使用基因本体分类和京都基因与基因组百科全书通路分析预测差异表达 miRNA 的功能。

结果

我们的结果表明，SA、UA 和 NCA 患者的循环 miRNA 表达谱存在差异。SA 和 UA 患者分别有 36 个和 161 个 miRNA 表达失调。通过逆转录定量聚合酶链反应对 miRNA 表达进行了验证。

结论

这些结果表明，循环 miRNAs 可能是 UA 的潜在生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65ac/7337223/477c1a58134c/cln-75-e1546-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65ac/7337223/85c5299b8f6f/cln-75-e1546-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65ac/7337223/29e0b37859fa/cln-75-e1546-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65ac/7337223/ca16eb4e0416/cln-75-e1546-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65ac/7337223/477c1a58134c/cln-75-e1546-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65ac/7337223/85c5299b8f6f/cln-75-e1546-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65ac/7337223/29e0b37859fa/cln-75-e1546-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65ac/7337223/ca16eb4e0416/cln-75-e1546-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65ac/7337223/477c1a58134c/cln-75-e1546-g004.jpg

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