Smecellato Fernanda Bombonato, Marsilli Lucas Ricardo Benfatti, Nakamura Julia Eico, Jordani Maria Cecília, Évora Paulo Roberto Barbosa, Castro-E-Silva Orlando
FAMEMA, Brazil.
Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirao Preto, SP, Brazil.
Acta Cir Bras. 2020;35(6):e202000603. doi: 10.1590/s0102-865020200060000003. Epub 2020 Jul 8.
Purpose To compare Fructose-1,6-Bisphosphate (FBP) to Histidine-Tryptophan-Ketoglutarate (HTK) in liver preservation at cold ischemia. Methods Male rats (Sprague-Dawley: 280-340g) divided into three groups (n=7): Control; Fructose-1,6-bisphosphate (FBP); Histidine-Tryptophan-Ketoglutarate (HTK). Animals underwent laparotomy-thoracotomy for perfusion of livers with saline. Livers were removed and deposited into solutions. Mitochondria were isolated to determine State 3 (S3), State 4 (S4), Respiratory Control Ratio (RCR) and Swelling (S). Liver enzymes (AST, ALT, LDH) were determined in solution. At tissue, Malondialdehyde (MDA) and Nitrate (NOx) were determined. All parameters were analyzed at 0.6 and 24 hours of hypothermic preservation. Statistics analysis were made by Mann-Whitney test (p<0.05). Results Regarding ALT, there was a difference between FBP-6h/HTK-6h, lower in HTK. Regarding AST, there was a significant difference between FBP-24h/HTK-24h, lower in FBP. Regarding NOx, there was a difference between 0h and 6h, as well as 0h and 24h for both solutions. Regarding S3, there was a significant difference in 24h compared to Control-0h for both solutions, and a significant difference between FBP-6h/FBP-24h. Regarding S4, there was a difference between Control-0h/HTK-24h and FBP-24h/HTK-24h, higher in HTK. There was a difference between Control-0h/FBP-24h for Swelling, higher in FBP. Conclusion Fructose-1,6-Bisphosphate showed better performance at nitrate and aspartate aminotransferase compared to histidine-tryptophan-ketoglutarate.
目的 比较果糖-1,6-二磷酸（FBP）和组氨酸-色氨酸-酮戊二酸（HTK）在肝脏冷缺血保存中的效果。方法 将雄性大鼠（Sprague-Dawley，体重280 - 340g）分为三组（n = 7）：对照组；果糖-1,6-二磷酸（FBP）组；组氨酸-色氨酸-酮戊二酸（HTK）组。动物接受剖腹-开胸手术，用生理盐水灌注肝脏。取出肝脏并置于相应溶液中。分离线粒体以测定状态3（S3）、状态4（S4）、呼吸控制率（RCR）和肿胀度（S）。测定溶液中的肝酶（AST、ALT、LDH）。在组织中测定丙二醛（MDA）和硝酸盐（NOx）。在低温保存0.6小时和24小时时分析所有参数。采用曼-惠特尼检验进行统计学分析（p < 0.05）。结果 关于ALT，FBP - 6小时/HTK - 6小时之间存在差异，HTK组较低。关于AST，FBP - 24小时/HTK - 24小时之间存在显著差异，FBP组较低。关于NOx，两种溶液在0小时与6小时以及0小时与24小时之间均存在差异。关于S3，两种溶液在24小时时与对照组0小时相比均存在显著差异，且FBP - 6小时/FBP - 24小时之间存在显著差异。关于S4，对照组0小时/HTK - 24小时与FBP - 24小时/HTK - 24小时之间存在差异，HTK组较高。关于肿胀度，对照组0小时/FBP - 24小时之间存在差异，FBP组较高。结论 与组氨酸-色氨酸-酮戊二酸相比，果糖-1,6-二磷酸在硝酸盐和天冬氨酸转氨酶方面表现更好。
