使用细菌病毒T7作为研究DNA甲基化的工具。 - Suppr | 超能文献

Use of bacterial virus T7 as a tool for the study of DNA methylation.

作者信息

Krüger D H, Schroeder C, Reuter M, Bickle T A, Bogdarina I G, Buryanov Y I

机构信息

Institute of Virology Humboldt University School of Medicine (Charité), Berlin, G.D.R.

出版信息

Gene. 1988 Dec 25;74(1):85-7. doi: 10.1016/0378-1119(88)90258-2.

DOI:10.1016/0378-1119(88)90258-2

Abstract

摘要

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Use of bacterial virus T7 as a tool for the study of DNA methylation.使用细菌病毒T7作为研究DNA甲基化的工具。

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2

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7

Methylation at overlapping dam (Gm6ATC) sites does not block cleavage by the NruI (TCGCGA) isoschizomer restriction endonucleases AmaI, SalDI and Sbo13I.重叠的dam（Gm6ATC）位点处的甲基化并不阻碍同裂酶限制性核酸内切酶AmaI、SalDI和Sbo13I（NruI，TCGCGA）的切割。

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Oligonucleotide duplexes containing CC(A/T)GG stimulate cleavage of refractory DNA by restriction endonuclease EcoRII.含有CC(A/T)GG的寡核苷酸双链体可刺激限制性内切酶EcoRII切割难切割的DNA。

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DNA methylation of bacterial viruses T3 and T7 by different DNA methylases in Escherichia coli K12 cells.大肠杆菌K12细胞中不同DNA甲基化酶对细菌病毒T3和T7的DNA甲基化作用。

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Theor Appl Genet. 1995 Oct;91(5):809-15. doi: 10.1007/BF00220964.

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Biochem J. 1990 Jan 15;265(2):309-20. doi: 10.1042/bj2650309.