Abe E, Ishimi Y, Takahashi N, Akatsu T, Ozawa H, Yamana H, Yoshiki S, Suda T
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
J Bone Miner Res. 1988 Dec;3(6):635-45. doi: 10.1002/jbmr.5650030609.
We have reported that the differentiation-inducing factor (DIF) is present in conditioned medium of mouse osteoblast-like cell (MC3T3-E1) cultures. In the present study, the DIF from conditioned medium of MC3T3-E1 cells was partially purified and its biologic activity was examined. The DIF was purified by monitoring the induction of phagocytic activity of mouse myeloblastic leukemia cells (M1). The DIF induced differentiation of not only M1 cells but also mouse myelomonocytic cells (WEHI-3). Furthermore, the DIF increased the in vitro bone-resorbing activity and the osteoclast number in mouse calvaria. The increases were inhibited by the addition of either salmon calcitonin or indomethacin. When mouse bone marrow cells were cultured with the DIF for 8 days, formation of osteoclast-like multinucleated cells was stimulated dose dependently. The DIF from MC3T3-E1 cells appeared to be different from interleukin-1 (IL-1), tumor necrosis factor (TNF), and transforming growth factor beta (TGF-beta). These results suggest that the DIF partially purified from osteoblast-like cell cultures stimulates osteoclastic bone resorption by promoting differentiation and fusion of osteoclast progenitors to form multinucleated osteoclasts.
我们曾报道，分化诱导因子（DIF）存在于小鼠成骨样细胞（MC3T3-E1）培养物的条件培养基中。在本研究中，对MC3T3-E1细胞条件培养基中的DIF进行了部分纯化，并检测了其生物学活性。通过监测小鼠骨髓性白血病细胞（M1）吞噬活性的诱导来纯化DIF。DIF不仅诱导M1细胞分化，还诱导小鼠骨髓单核细胞（WEHI-3）分化。此外，DIF增加了小鼠颅骨的体外骨吸收活性和破骨细胞数量。添加鲑鱼降钙素或吲哚美辛可抑制这种增加。当小鼠骨髓细胞与DIF一起培养8天时，破骨细胞样多核细胞的形成受到剂量依赖性刺激。MC3T3-E1细胞的DIF似乎与白细胞介素-1（IL-1）、肿瘤坏死因子（TNF）和转化生长因子β（TGF-β）不同。这些结果表明，从成骨样细胞培养物中部分纯化的DIF通过促进破骨细胞祖细胞的分化和融合以形成多核破骨细胞来刺激破骨性骨吸收。
