Nature. 2020 Jul;583(7817):590-595. doi: 10.1038/s41586-020-2496-1. Epub 2020 Jul 15.
Ageing is characterized by a progressive loss of physiological integrity, leading to impaired function and increased vulnerability to death. Despite rapid advances over recent years, many of the molecular and cellular processes that underlie the progressive loss of healthy physiology are poorly understood. To gain a better insight into these processes, here we generate a single-cell transcriptomic atlas across the lifespan of Mus musculus that includes data from 23 tissues and organs. We found cell-specific changes occurring across multiple cell types and organs, as well as age-related changes in the cellular composition of different organs. Using single-cell transcriptomic data, we assessed cell-type-specific manifestations of different hallmarks of ageing-such as senescence, genomic instability and changes in the immune system. This transcriptomic atlas-which we denote Tabula Muris Senis, or 'Mouse Ageing Cell Atlas'-provides molecular information about how the most important hallmarks of ageing are reflected in a broad range of tissues and cell types.
衰老是指生理完整性的逐渐丧失，导致功能受损和死亡风险增加。尽管近年来取得了快速进展，但许多潜在的分子和细胞过程仍知之甚少。为了更好地了解这些过程，我们在这里生成了一个横跨 Mus musculus 生命周期的单细胞转录组图谱，其中包括来自 23 种组织和器官的数据。我们发现，多个细胞类型和器官中存在细胞特异性变化，不同器官的细胞组成也存在与年龄相关的变化。我们使用单细胞转录组数据评估了不同衰老标志在不同细胞类型中的表现，例如衰老、基因组不稳定性和免疫系统变化。这个转录组图谱——我们称之为 Tabula Muris Senis，或“老鼠衰老细胞图谱”——提供了关于衰老的最重要标志如何在广泛的组织和细胞类型中反映的分子信息。
