Department of Neurology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, China.
The Neurological Institute of Jiangxi Province, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, China.
Front Immunol. 2020 Jun 25;11:1329. doi: 10.3389/fimmu.2020.01329. eCollection 2020.
To investigate the frequencies and numbers of invariant natural killer T (iNKT) cells and γδT cells in the peripheral blood of patients with the Parkinson's disease (PD), and to examine their association with the PD severity. Peripheral blood samples from 47 PD patients (PD group) and 47 age-matched healthy control subjects (HC group) were collected. The frequencies and the absolute cell numbers were analyzed by flow cytometry. Mann-Whitney -test was used to test the difference between two groups, where < 0.05 was considered as significant. An ordered probit regression method was used to examine the association of the iNKT and γδT cells with severity of PD. Patients in the PD group showed significantly lower frequencies (0.039 vs. 0.139%; = 0) and cell counts (308/mL vs. 1,371/mL; = 0) of iNKT cells compared to the HC group. Moreover, the percentages and absolute numbers of γδT cells were significantly decreased in the PD group compared to the HC group (3.69 vs. 7.95% and 30/μL vs. 66/μL; = 0). The iNKT cells were significantly reduced in PD patients with higher Unified Parkinson's Disease Rating Scale (UPDRS) scores or cognitive decline. Cell frequencies and absolute numbers of iNKT cells and γδT cells are significantly reduced in the peripheral blood samples of PD patients. Patients with high UPDRS scores or cognitive decline also showed significant reduction of iNKT cells. Our results suggest that iNKT cells and γδT cells may contribute to the development of PD.
为了研究帕金森病（PD）患者外周血中不变自然杀伤 T（iNKT）细胞和γδT 细胞的频率和数量，并探讨其与 PD 严重程度的关系。采集了 47 例 PD 患者（PD 组）和 47 名年龄匹配的健康对照者（HC 组）的外周血样本。采用流式细胞术分析频率和绝对细胞数。采用 Mann-Whitney U 检验比较两组间差异， ＜0.05 为差异有统计学意义。采用有序概率回归方法分析 iNKT 和 γδT 细胞与 PD 严重程度的关系。PD 组患者 iNKT 细胞频率（0.039 比 0.139%， ＝0）和细胞计数（308/mL 比 1371/mL， ＝0）明显低于 HC 组。此外，与 HC 组相比，PD 组患者 γδT 细胞的百分比和绝对数明显减少（3.69 比 7.95%和 30/μL 比 66/μL， ＝0）。PD 患者 UPDRS 评分较高或认知功能下降时，iNKT 细胞明显减少。PD 患者外周血中 iNKT 细胞和 γδT 细胞的频率和绝对数明显减少。UPDRS 评分较高或认知功能下降的患者 iNKT 细胞也明显减少。我们的研究结果表明，iNKT 细胞和 γδT 细胞可能参与了 PD 的发生发展。
