Zhao Congcong, Xie Yuanchen, Zhou Xiaoshan, Zhang Qiao, Wang Na
College of Public Health, Zhengzhou University, No. 100 Science Avenue, High-tech Zone, Henan 450001, China.
Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute, Karolinska University Hospital, Huddinge, Stockholm 141 86, Sweden.
Toxicol Res (Camb). 2020 May 20;9(3):302-307. doi: 10.1093/toxres/tfaa031. eCollection 2020 Jun.
To explore the genetic damage caused by different tar levels in the human body.
The subjects were divided into high, medium and low (12 mg, 8 mg, 5 mg) tar groups according to the tar levels. Nonsmoking populations served as a control group. 2 ml of peripheral blood was collected on the 10th day after morning fasting. Oxidative and genetic toxicological damage indicators were analysed with enzyme-linked immunosorbent assay, cytokinesis-block micronucleus assay in human lymphocyte and single cell gel electrophoresis.
The distribution of hOGG1 concentration was significantly different within all groups, < 0.01. The concentrations of cotinine, 8-OHdG and Rap-2b were significantly differences between control and medium tar group, control and high tar group, low and medium tar group and low and high tar group, respectively, < 0.05. The level of PAH-DNA adducts was not significantly changed in the middle tar group and high tar group, 0.05. The level of CRP was significantly changed between control and high tar group, low and high tar group and medium and high tar group, respectively, < 0.0001. The rate of comet tailing was significantly different between all groups. The rate of micronucleus cells was not significantly different between all groups.
The increase of tar content could increase the DNA damage to a certain extent, so the intake of tar content should be monitored.
探讨人体中不同焦油水平所造成的基因损伤。
根据焦油水平将受试者分为高、中、低（12毫克、8毫克、5毫克）焦油组。非吸烟人群作为对照组。在早晨空腹10天后采集2毫升外周血。采用酶联免疫吸附测定、人淋巴细胞胞质分裂阻滞微核试验和单细胞凝胶电泳分析氧化和遗传毒理学损伤指标。
所有组内hOGG1浓度分布存在显著差异，<0.01。可替宁、8-羟基脱氧鸟苷和Rap-2b的浓度在对照组与中焦油组、对照组与高焦油组、低焦油组与中焦油组以及低焦油组与高焦油组之间分别存在显著差异，<0.05。中焦油组和高焦油组中多环芳烃-DNA加合物水平无显著变化，>0.05。CRP水平在对照组与高焦油组、低焦油组与高焦油组以及中焦油组与高焦油组之间分别有显著变化，<0.0001。所有组之间彗星拖尾率存在显著差异。所有组之间微核细胞率无显著差异。
焦油含量的增加可在一定程度上增加DNA损伤，因此应监测焦油含量的摄入量。
