Upadhyay Arpan, Chakravarty Atish Kumar, De Sachinandan, Gupta Ashok Kumar, Singh Avtar, Sakthivel Selvan A
Krishi Vigyan Kendra, Jhansi, Banda University of Agriculture and Technology, Banda.
Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal- 132001, Haryana, India.
Iran J Biotechnol. 2019 Dec 1;17(4):e2084. doi: 10.30498/IJB.2019.102188. eCollection 2019 Dec.
The bovine gene is one of the potential candidate genes governing milk fat synthesis.
Identification of single nucleotide polymorphisms (SNP) in the targeted region of gene and their effect on expected breeding values (EBV) of first lactation milk production traits viz. fat %, fat yield and 305 days milk yield in Karan Fries (KF) breeding bulls were sought.
A tetra-primer ARMS PCR technique was adapted to genotype an SNP, g.36,175,805C>T located on 5' flanking region of gene. The relationship between EBV of milk production traits and polymorphic locus of gene was assessed.
Three kinds of genotype (CC, CT, and TT) with respect to g.36,175,805C>T SNP locus were observed. The identified SNP had significant (P<0.05) influence on EBV of fat % (EBV-FP). The KF bulls with CC and CT genotype had comparatively higher EBV-FP than the bulls with the TT genotype. The substitution of "C" allele by "T" allele led to a decrease of 0.0045 % in the EBV-FP.
The identified SNP was significantly associated with EBV-FP, thus it may be utilized as a molecular marker for developing marker-assisted selection strategy to enhance the milk fat content in KF cattle population.
牛的[基因名称]基因是调控乳脂肪合成的潜在候选基因之一。
在[基因名称]基因的目标区域鉴定单核苷酸多态性（SNP）及其对卡兰弗里斯（KF）种公牛头胎产奶性状预期育种值（EBV）的影响，即乳脂肪率、乳脂肪产量和305天产奶量。
采用四引物扩增受阻突变系统聚合酶链反应（tetra-primer ARMS PCR）技术对位于[基因名称]基因5'侧翼区域的一个SNP（g.36,175,805C>T）进行基因分型。评估了产奶性状的EBV与[基因名称]基因多态性位点之间的关系。
在g.36,175,805C>T SNP位点观察到三种基因型（CC、CT和TT）。所鉴定的SNP对乳脂肪率的EBV（EBV-FP）有显著（P<0.05）影响。CC和CT基因型的KF公牛的EBV-FP相对高于TT基因型的公牛。“C”等位基因被“T”等位基因取代导致EBV-FP降低0.0045%。
所鉴定的SNP与EBV-FP显著相关，因此它可作为分子标记用于制定标记辅助选择策略，以提高KF牛群的乳脂肪含量。
