Molecular Genetics Lab, Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal, Haryana, India.
Department of Animal Science, Salale University, Fitche, Ethiopia.
Vet Med Sci. 2022 Nov;8(6):2593-2604. doi: 10.1002/vms3.924. Epub 2022 Sep 5.
Bovine mastitis continues to remain as the most challenging disease in dairy cattle, as a result improvement of selection methods has great economic relevance while a deeper understanding of the genetic mechanisms regulating milk production traits and mastitis is of general scientific interest.
This study aimed to evaluate the association of SNPs of the LAP3 and SIRT1 genes with estimated breeding values (EBVs) of milk production traits and clinical mastitis in dairy cattle of Indian origin.
DNA samples from 263 animals (Sahiwal and Karan Fries cattle) were genotyped by PCR-RFLP to assess their pattern of genetic variation. EBVs of milk production traits and phenotypic records of incidences of clinical mastitis were used for association analysis.
A total of 9 SNPs were identified, including three (rs110932626: A>G, rs716493845: C>T and rs43702363: C>T) in intron 12, four (g.24904G>C, rs110839532: G>T, rs43702361: T>C and rs41255599: C>T) in exon 13 and within 3'UTR of LAP3 gene and two (rs110250233: G>A and rs42140046: C>G) in the promoter region of SIRT1 gene. Eight of these identified SNPs were chosen for subsequent genotyping and association analyses. Association analysis revealed that SNP rs41255599: C>T was significantly associated with lactation milk yield, 305-day milk yield, 305-day fat yield, 305-day solid not fat yield, lactation length and incidence of clinical mastitis (p < 0.05) in Sahiwal cattle. For Karan Fries cattle, two SNPs including rs110932626: A>G and rs43702363: C>T showed significant association with 305-day milk yield.
Overall, these findings provide evidence for association of the LAP3 gene with milk production traits and clinical mastitis in dairy cattle, indicating the potential role of LAP3 variants in these traits.
奶牛乳腺炎仍然是奶牛养殖业中最具挑战性的疾病，因此，改进选择方法具有重要的经济意义，而深入了解调节牛奶生产性状和乳腺炎的遗传机制则具有普遍的科学意义。
本研究旨在评估 LAP3 和 SIRT1 基因的 SNP 与印度来源奶牛的产奶性状和临床乳腺炎的估计育种值（EBV）之间的关联。
对 263 头动物（萨希瓦尔牛和卡兰弗莱牛）的 DNA 样本进行 PCR-RFLP 基因分型，以评估其遗传变异模式。使用产奶性状 EBV 和临床乳腺炎表型记录进行关联分析。
共发现 9 个 SNP，其中 3 个（rs110932626：A>G、rs716493845：C>T 和 rs43702363：C>T）位于内含子 12，4 个（g.24904G>C、rs110839532：G>T、rs43702361：T>C 和 rs41255599：C>T）位于 LAP3 基因的外显子 13 内和 3'UTR 内，另外 2 个（rs110250233：G>A 和 rs42140046：C>G）位于 SIRT1 基因的启动子区域。其中 8 个鉴定的 SNP 被选择用于随后的基因分型和关联分析。关联分析显示，Sahiwal 牛中 SNP rs41255599：C>T 与泌乳期乳产量、305 天乳产量、305 天脂肪产量、305 天非脂固形物产量、泌乳期长度和临床乳腺炎发生率显著相关（p<0.05）。对于 Karan Fries 牛，包括 rs110932626：A>G 和 rs43702363：C>T 在内的两个 SNP 与 305 天乳产量显著相关。
总体而言，这些发现为 LAP3 基因与奶牛产奶性状和临床乳腺炎之间的关联提供了证据，表明 LAP3 变异在这些性状中可能发挥作用。
