Malik Amna, Butt Tayyab Ashfaq, Naqvi Syed Tatheer Alam, Yousaf Sohail, Qureshi Muhammad Kamran, Zafar Mazhar Iqbal, Farooq Ghazanfar, Nawaz Ismat, Iqbal Mazhar
Department of Environmental Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Civil Engineering, College of Engineering, University of Hail, Hail, Saudi Arabia.
Heliyon. 2020 Jul 4;6(7):e04188. doi: 10.1016/j.heliyon.2020.e04188. eCollection 2020 Jul.
Rapid industrialization and increasing population are continuously adding contaminants to our environment. Among those, heavy metals are considered to be one of the serious threats to the ecosystem due to their persistent nature. Microbe assisted phytoremediation is an effective tool for metal remediation as microbes enhance the metal availability and uptake to the host plants or reduce it by binding them intracellularly or extracellularly. An endophytic fungus, , was isolated from L. plant growing in the lead (Pb) contaminated soil of an industrial area. This is the first study citing , as a root endophyte of L. This endophytic fungus was found to be tolerant to high concentration of Pb i.e., 1500 mg L, when tested . Wheat ( L.) seedlings were infected by and Pb tolerance was observed. With the fungal inoculation plants cumulative growth and total chlorophyll content increased by 24% and 18%, respectively as compared to their respective non-inoculated controls at 1000 mg kg Pb. Similary, 50% more Pb accumulation was measured in the shoots of fungal inoculated plants at 1500 mg kg Pb as compared to control. Thus, the results of the present study suggest that mutualism with endophytic fungi can improve the survival of host plants in metal contaminated soils, additionally it can also assist the phytoextraction of heavy metals from polluted sites by increasing their uptake by the host plants.
快速工业化和人口增长不断给我们的环境增添污染物。其中，重金属因其持久性被认为是对生态系统的严重威胁之一。微生物辅助植物修复是一种有效的金属修复工具，因为微生物可提高金属对宿主植物的有效性和吸收量，或者通过细胞内或细胞外结合金属来降低其有效性。从生长在工业区铅（Pb）污染土壤中的羽扇豆（L.）植物中分离出一种内生真菌。这是首次将该内生真菌作为羽扇豆的根内生菌进行引用的研究。经测试，发现这种内生真菌对高浓度铅（即1500毫克/升）具有耐受性。用该真菌感染小麦（L.）幼苗并观察其对铅的耐受性。在1000毫克/千克铅的条件下，与各自未接种的对照相比，接种真菌的植物累积生长量和总叶绿素含量分别增加了24%和18%。同样，在1500毫克/千克铅的条件下，接种真菌的植物地上部分的铅积累量比对照多50%。因此，本研究结果表明，与内生真菌的共生关系可以提高宿主植物在金属污染土壤中的存活率，此外，它还可以通过增加宿主植物对重金属的吸收来协助从污染场地植物提取重金属。
