Kolho Kaija-Leena, Alfthan Henrik
Children´s Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Helsinki University Hospital Laboratory (HUSLAB), Helsinki University Hospital, Helsinki, Finland.
Scand J Gastroenterol. 2020 Sep;55(9):1024-1027. doi: 10.1080/00365521.2020.1794026. Epub 2020 Jul 15.
Fecal calprotectin is a valued surrogate marker for intestinal inflammation. It has been argued that calprotectin levels are higher in early age than in later life hampering the use of calprotectin in young children.
To study age-related variation, we used data from our laboratory information system on consecutive, unselected fecal calprotectin measurements from 2014 to 2017 in all children aged 0 to 18 years. From each individual, the first measurement was included and repeated measurements were excluded. Fecal calprotectin was quantitated in the major clinical laboratory in southern Finland, HUSLAB with an ELISA kit from Calpro AS (Calpro/Calprolab, Oslo, Norway). Currently, the assay is performed on two automatic pipetting analysers (Dynex DS2, Chantilly, USA) according to the instructions of the manufacturer.
There were altogether 11,255 fecal calprotectin results from as many children. The median level of fecal calprotectin was 51 mg/kg in infants < 1 year of age (95 percentile 648 mg/kg; = 239). This was 3-4-fold higher when compared to yearly age groups from 1 to 10 years (total number of children included 5,691). Across yearly age groups from 11 to 18, the median values varied from 11 to 19 mg/kg (total number of included children 5,325). The proportion of samples above the routine cut-off for an elevated concentration >100 mg/kg increased with increasing age.
Fecal calprotectin values in children beyond the first year of life are in general low and comparable in children and adolescents.
粪便钙卫蛋白是肠道炎症的重要替代标志物。有人认为，钙卫蛋白水平在幼年时高于晚年，这限制了其在幼儿中的应用。
为研究年龄相关差异，我们使用了实验室信息系统中2014年至2017年对所有0至18岁儿童连续、未经筛选的粪便钙卫蛋白测量数据。从每个个体中纳入首次测量结果，排除重复测量结果。粪便钙卫蛋白在芬兰南部的主要临床实验室HUSLAB中使用来自Calpro AS（Calpro/Calprolab，挪威奥斯陆）的ELISA试剂盒进行定量。目前，该检测根据制造商的说明在两台自动移液分析仪（Dynex DS2，美国尚蒂伊）上进行。
共有来自同样数量儿童的11255份粪便钙卫蛋白检测结果。1岁以下婴儿的粪便钙卫蛋白中位数水平为51mg/kg（第95百分位数为648mg/kg；n = 239）。与1至10岁的各年龄组相比，该水平高出3至4倍（纳入儿童总数为5691）。在11至18岁的各年龄组中，中位数从11mg/kg至19mg/kg不等（纳入儿童总数为5325）。浓度高于常规临界值>100mg/kg的样本比例随年龄增长而增加。
1岁以后儿童的粪便钙卫蛋白值总体较低，在儿童和青少年中相当。
