Abou-Seri Hanan M, Ibrahim Asmaa, Zahran Fatima
Parasitology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Genetic Engineering and Biotechnology Research Institute, University of Sadat City (GEBRI, USC), Sadat, Egypt.
Iran J Parasitol. 2022 Oct-Dec;17(4):488-496. doi: 10.18502/ijpa.v17i4.11275.
Giardiasis is one of the leading causes of diarrhea, particularly among children under the age of five in developing countries. Fecal calprotectin (FC) is an important biomarker for diagnosis and monitoring of inflammtory bowel disease, but other diagnoses should be considered in light of its elevation. We aimed to evaluate FC level in patients diagnosed with giardiasis and elucidate a possible correlation between genotypes and FC levels.
Overall, 120 fecal samples were collected from children aged 4-12 years and tested for giardiasis by light microscopy. Out of which, 50 samples were enrolled within two groups: group I "cases" and group II "controls" and then subjected to PCR amplification, sequencing of the beta-giardin (bg) gene of the parasite, and FC evaluation.
Assemblage B was identified in 75%, and assemblage A in 25% of samples. FC levels were statistically elevated in "group I" in comparison to "group II". Likewise, there was a statistically significant difference between FC levels in patients infected with assemblage A and assemblage B with a mean of 114 μg/gm and 202 μg/gm, respectively.
The study highlighted the possible association between genotype B and elevated FC levels, further detailed studies are necessary to clarify these finding.
贾第虫病是腹泻的主要病因之一，在发展中国家，五岁以下儿童中尤为常见。粪便钙卫蛋白（FC）是诊断和监测炎症性肠病的重要生物标志物，但鉴于其升高情况，应考虑其他诊断。我们旨在评估被诊断为贾第虫病患者的FC水平，并阐明基因型与FC水平之间可能存在的相关性。
总体而言，从4至12岁儿童中收集了120份粪便样本，通过光学显微镜检测贾第虫病。其中，50份样本被纳入两组：第一组“病例组”和第二组“对照组”，然后进行PCR扩增、寄生虫β-贾第虫（bg）基因测序以及FC评估。
75%的样本鉴定为B群，25%为A群。与“第二组”相比，“第一组”的FC水平在统计学上有所升高。同样，感染A群和B群患者的FC水平之间存在统计学上的显著差异，平均分别为114μg/gm和202μg/gm。
该研究突出了B基因型与FC水平升高之间可能存在的关联，需要进一步详细研究以阐明这些发现。
