Central R&D Center, Bioresources and Technology (B&Tech) Co., Ltd., Gwangju 61239, Korea.
College of Korean Medicine, Dongshin University, 185 Geonjae-ro, Naju-si, Jeollanam-do 58245, Korea.
Int J Mol Sci. 2020 Jul 14;21(14):4976. doi: 10.3390/ijms21144976.
We investigated the time-dependent deleterious ocular changes induced by urban particulate matter (UPM) in vitro and in vivo. UPM treatment decreased human corneal epithelial cell migration and survival. Fluorescein scores were consistently increased by UPM application for 16 weeks. One week of rest at 2 or 4 weeks led to a recovery trend, whereas two weeks of rest at 8 weeks induced no change. UPM treatment decreased the tear film break-up time at 2 weeks, which was thereafter maintained until 16 weeks. No changes were found after periods of rest. UPM-treated eyes exhibited greater corneal epithelium thickness than normal eyes at 2 weeks, which recovered to normal at 4 and 8 weeks and was significantly decreased at 16 weeks. Apoptotic cell number in the epithelium was increased at 2 weeks, which remained constant except at 8 weeks. IL-6 expression in the cornea of the right eye continually increased for 16 weeks, and significant recovery was only observed at 8 weeks after 2 weeks of rest. Ocular pressure was significantly increased in the right eye at 12 and 16 weeks. Topical UPM application to the eye induced deleterious changes to various closely related parts of the eye.
我们研究了城市颗粒物(UPM)在体外和体内引起的时间依赖性有害眼部变化。UPM 处理降低了人角膜上皮细胞的迁移和存活。荧光素评分在 UPM 应用 16 周内持续增加。2 或 4 周休息 1 周导致恢复趋势,而 8 周休息 2 周则没有变化。UPM 处理在 2 周时降低了泪膜破裂时间,此后一直持续到 16 周。休息期后没有发现变化。在 2 周时,接受 UPM 治疗的眼睛的角膜上皮厚度比正常眼睛大,在 4 周和 8 周时恢复正常,在 16 周时显著降低。上皮细胞中的凋亡细胞数量在 2 周时增加,除了在 8 周时保持不变。右眼的 IL-6 表达持续增加了 16 周,只有在 2 周休息 8 周后才观察到显著恢复。右眼眼压在 12 和 16 周时显著升高。眼部局部应用 UPM 会对眼睛的各个密切相关部位造成有害变化。