Department of Biochemistry and Cell Biology, Federal University of Viçosa, Viçosa, Brazil.
Recognizing Biology Laboratory, Bioscience and Biotechnology Center, North Fluminense State University, Rio de Janeiro, Brazil.
Nat Prod Res. 2021 Dec;35(23):5554-5558. doi: 10.1080/14786419.2020.1795656. Epub 2020 Jul 16.
The present study investigated the possibility of apoptosis-inducing activity in human leukemia U-937 and THP-1 cells by the flavonoid morin. The treatments were evaluated by using the MTT and LDH assays; analysis of mitochondrial membrane potential (ΔΨm) was evaluated by flow cytometry, cell death by apoptosis was confirmed by fluorescence microscopy and by assessing the activity of caspases-3 and -6. The data indicated that the flavonoid morin has promoted a decrease in cell viability in a concentration-dependent way for both of the cancerous cell lines. An increase in the percentage of cell death caused by apoptosis was associated to a potential alteration in the mitochondrial membrane (ΔΨm) suggesting the involvement of cell death in intrinsic apoptotic pathways. Activation of caspases-3 and -6 confirmed the presence of apoptotic activity from morin. The results reinforce the antileukemic potential of flavonol morin.
本研究探讨了黄酮醇桑色素对人白血病 U-937 和 THP-1 细胞诱导凋亡活性的可能性。通过 MTT 和 LDH 检测评估处理效果；通过流式细胞术分析线粒体膜电位 (ΔΨm)，通过荧光显微镜和检测 caspase-3 和 -6 的活性来确认细胞死亡的凋亡情况。数据表明，黄酮醇桑色素能够促进两种癌细胞系的细胞活力以浓度依赖的方式下降。凋亡引起的细胞死亡百分比增加与线粒体膜（ΔΨm）的潜在改变相关，提示细胞死亡涉及内在凋亡途径。caspase-3 和 -6 的激活证实了桑色素具有诱导凋亡的活性。这些结果增强了黄酮醇桑色素的抗白血病潜力。
