泰国枳（Maclura cochinchinensis（Lour.）Corner）提取物和化合物的生物活性。
Biological activities of extracts and compounds from Thai Kae-Lae (Maclura cochinchinensis (Lour.) Corner).
机构信息
Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
出版信息
BMC Complement Med Ther. 2023 Jun 9;23(1):191. doi: 10.1186/s12906-023-03979-w.
BACKGROUND AND AIMS
The purpose of this study was to investigate the biological properties of Kae-Lae (Maclura cochinchinensis (Lour.) Corner), a traditional medicinal plant used in Ayurvedic recipes in Thailand. To achieve this objective, heartwood samples were collected from 12 sources across Thailand. Fractional extracts (n-hexane, ethyl acetate, and ethanol) and the dominant compounds (morin, resveratrol, and quercetin) were examined for their abilities on cytotoxicity, antioxidant, anti-inflammation, and antileukaemic activity (Wilms' tumour 1 protein was used as a well-known biomarker for leukaemic cell proliferation).
METHODS
The study used MTT to assess cytotoxicity in leukaemic cells (K562, EoL-1, and KG-1a). Antioxidant activities were evaluated using ABTS, DPPH, and FRAP assays. The anti-inflammatory activity was investigated by detecting IL-2, TNF-α, and NO using appropriate detection kits. Wilms' tumour 1 protein expression was measured by Western blotting to determine the anti-leukaemic activity. The inhibition of cell migration was also analyzed to confirm anticancer progression.
RESULTS
Among the tested extract fraction, ethyl acetate No. 001 displayed strong cytotoxicity specifically in EoL-1 cells, while n-hexane No. 008 demonstrated this effect in three cell lines. Resveratrol, on the other hand, displayed cytotoxicity in all the tested cells. Additionally, the three major compounds, morin, resveratrol, and quercetin, exhibited significant antioxidant and anti-inflammatory properties. In particular, resveratrol demonstrated a noteworthy decreased Wilms' tumour 1 protein expression and a reduction in cell proliferation across all cells. Moreover, ethyl acetate No. 001, morin, and resveratrol effectively inhibited MCF-7 cell migration. None of these compounds showed any impact on red blood cell haemolysis.
CONCLUSION
Based on these findings, it can be concluded that Kae-Lae has promising chemotherapeutic potential against leukaemic cells, with fractional extracts (ethyl acetate and n-hexane) and resveratrol exhibiting the most potent cytotoxic, antioxidant, anti-inflammatory, and anti-cell migration activities.
背景与目的
本研究旨在探究泰国阿育吠陀配方中使用的传统药用植物嘉兰（Maclura cochinchinensis（Lour.）Corner）的生物学特性。为此，从泰国 12 个不同的地点采集心材样本。对其正丁醇、乙酸乙酯和乙醇级分提取物（fractional extracts）以及主要化合物（morin、resveratrol 和 quercetin）的细胞毒性、抗氧化、抗炎和抗白血病活性（Wilms' tumour 1 protein 被用作白血病细胞增殖的一个众所周知的生物标志物）进行了检测。
方法
本研究使用 MTT 评估白血病细胞（K562、EoL-1 和 KG-1a）的细胞毒性。使用 ABTS、DPPH 和 FRAP 测定法评估抗氧化活性。通过使用适当的检测试剂盒检测 IL-2、TNF-α 和 NO 来研究抗炎活性。通过 Western blot 检测 Wilms' tumour 1 protein 表达来确定抗白血病活性。还分析了细胞迁移抑制作用以证实抗癌进展。
结果
在所测试的提取物级分中，乙酸乙酯 001 号对 EoL-1 细胞具有强烈的细胞毒性，而正丁醇 008 号对三种细胞系均显示出这种作用。另一方面，resveratrol 在所有测试的细胞中均显示出细胞毒性。此外，morin、resveratrol 和 quercetin 这三种主要化合物具有显著的抗氧化和抗炎特性。特别是，resveratrol 在所有细胞中均表现出显著降低的 Wilms' tumour 1 protein 表达和细胞增殖减少。此外，乙酸乙酯 001 号、morin 和 resveratrol 有效抑制 MCF-7 细胞迁移。这些化合物均未对红细胞溶血产生任何影响。
结论
根据这些发现，可以得出结论，嘉兰具有治疗白血病细胞的潜在化学治疗作用，其级分提取物（乙酸乙酯和正丁醇）和 resveratrol 表现出最强的细胞毒性、抗氧化、抗炎和抗细胞迁移活性。
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