Fu Xueqing, Liu Hang, Hassani Danial, Peng Bowen, Yan Xin, Wang Yuting, Wang Chen, Li Ling, Liu Pin, Pan Qifang, Zhao Jingya, Qian Hongmei, Sun Xiaofen, Tang Kexuan
Joint International Research Laboratory of Metabolic & Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai, China.
Front Plant Sci. 2020 Jun 26;11:950. doi: 10.3389/fpls.2020.00950. eCollection 2020.
The phytohormone Abscisic acid (ABA) regulates plant growth, development, and responses to abiotic stresses, including senescence, seed germination, cold stress and drought. Several kinds of researches indicate that exogenous ABA can enhance artemisinin content in . Some transcription factors related to ABA signaling are identified to increase artemisinin accumulation through activating the artemisinin synthase genes. However, no prior study on ABA transporter has been performed in . Here, we identified a pleiotropic drug resistance (PDR) transporter gene from . was expressed mainly in roots, leaves, buds, and trichomes. GUS activity is primarily observed in roots and the vascular tissues of young leaves in : transgenic plants. When was transferred into yeast AD12345678, yeasts expressing accumulated more ABA than the control. The overexpressing plants showed higher artemisinin content and stronger drought tolerance. Besides, the expression of in OE- plants showed more sensitivity to exogenous ABA than that in both wild-type and i plants. According to these results, they strongly suggest that AaABCG40 is involved in ABA transport in .
植物激素脱落酸（ABA）调控植物的生长、发育以及对非生物胁迫的响应，包括衰老、种子萌发、冷胁迫和干旱。多项研究表明，外源ABA可提高青蒿素含量。一些与ABA信号传导相关的转录因子通过激活青蒿素合成酶基因来增加青蒿素积累。然而，此前尚未在青蒿中开展过关于ABA转运蛋白的研究。在此，我们从青蒿中鉴定出一个多药耐药（PDR）转运蛋白基因。AaABCG40主要在根、叶、芽和腺毛中表达。在AaABCG40转基因青蒿植株中，GUS活性主要在根和幼叶的维管组织中观察到。当AaABCG40转入酵母AD12345678时，表达AaABCG40的酵母积累的ABA比对照更多。过表达AaABCG40的植株显示出更高的青蒿素含量和更强的耐旱性。此外，在OE-AaABCG40植株中AaABCG40的表达对外源ABA的敏感性高于野生型和i植株。根据这些结果，强烈表明AaABCG40参与青蒿中的ABA转运。
