Fu Xueqing, Shi Pu, He Qian, Shen Qian, Tang Yueli, Pan Qifang, Ma Yanan, Yan Tingxiang, Chen Minghui, Hao Xiaolong, Liu Pin, Li Ling, Wang Yuliang, Sun Xiaofen, Tang Kexuan
Joint International Research Laboratory of Metabolic and Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong UniversityShanghai, China.
Front Plant Sci. 2017 May 8;8:723. doi: 10.3389/fpls.2017.00723. eCollection 2017.
Artemisinin, a sesquiterpenoid endoperoxide, isolated from the plant L., is widely used in the treatment of malaria. Another sesquiterpenoid, β-caryophyllene having antibiotic, antioxidant, anticarcinogenic and local anesthetic activities, is also presented in . The role played by sesquiterpene transporters in trichomes and accumulation of these metabolites is poorly understood in and in trichomes of other plant species. We identified , encoding a pleiotropic drug resistance (PDR) transporter located to the plasma membrane from . Expression of is tissue-specifically and developmentally regulated in . GUS activity is primarily restricted to T-shaped trichomes of old leaves and roots of transgenic plants expressing : . The level of β-caryophyllene was decreased in transgenic plants expressing -RNAi while transgenic plants expressing increased levels of accumulated higher levels of β-caryophyllene. When AaPDR3 was expressed in transformed yeast, yeasts expressing accumulated more β-caryophyllene, rather than germacrene D and β-farnesene, compared to the non-expressing control.
青蒿素是一种从植物黄花蒿中分离出的倍半萜内过氧化物，被广泛用于治疗疟疾。另一种倍半萜β-石竹烯具有抗生素、抗氧化、抗癌和局部麻醉活性，也存在于黄花蒿中。在黄花蒿以及其他植物物种的毛状体中，倍半萜转运体在这些代谢产物的积累中所起的作用尚不清楚。我们从黄花蒿中鉴定出一个编码定位于质膜的多药耐药（PDR）转运体的基因AaPDR3。AaPDR3的表达在黄花蒿中受到组织特异性和发育调控。在表达AaPDR3::GUS的转基因黄花蒿植物中，GUS活性主要局限于老叶和根的T形毛状体。在表达AaPDR3-RNAi的转基因黄花蒿植物中，β-石竹烯水平降低，而在表达AaPDR3水平升高的转基因黄花蒿植物中，β-石竹烯积累水平更高。当AaPDR3在转化酵母中表达时，与未表达的对照相比，表达AaPDR3的酵母积累了更多的β-石竹烯，而不是杜松烯D和β-法尼烯。
