Wang Yu-Jing, Ke Min
Department of Ophthalmology, Zhongnan Hospital of Wuhan University, Wuhan 430060, Hubei, China.
J Ophthalmol. 2020 Jun 30;2020:7516286. doi: 10.1155/2020/7516286. eCollection 2020.
In vivo confocal laser scanning microscopy (CLSM) is an emerging diagnostic tool allowing fast and easy microscopic tissue examination. For the diagnostics of pathological eyelid margin lesions, the knowledge of the normal eyelid margin is essential.
We examined 18 eyelid margins of healthy humans using the in vivo CLSM device and 10 samples of healthy eyelid margins from donor sites with ex vivo CLSM and compared the findings to the corresponding histological sections of donor sites. Cross-section images of different depths and depths of different skin appendages were measured.
The depth observed by in vivo CLSM is less than 150 m into the eyelid. Images of the epidermis and superficial dermis skin, appendages including hair follicle, and sebaceous catheters can be captured associated with histopathology and ex vivo confocal microscopy. In correlation with histopathology, we identified different layers of the eyelid margin, different layers of the epidermis, and skin appendages by ex vivo confocal microscopy.
The study offers an overview of the in vivo confocal microscopy human eyelid margin characteristics in comparison to the standard histological examination and confirms that in vivo CLSM could not observe the meibomian gland acini structure.
活体共聚焦激光扫描显微镜（CLSM）是一种新兴的诊断工具，可实现快速、简便的微观组织检查。对于病理性睑缘病变的诊断，了解正常睑缘情况至关重要。
我们使用活体CLSM设备检查了18例健康人的睑缘，并使用离体CLSM检查了来自供体部位的10份健康睑缘样本，并将结果与供体部位相应的组织学切片进行比较。测量了不同深度和不同皮肤附属器深度的横截面图像。
活体CLSM观察到的深度在睑缘内小于150μm。可捕获表皮和浅表真皮皮肤、包括毛囊和皮脂腺导管在内的附属器的图像，这些图像与组织病理学和离体共聚焦显微镜检查相关。通过离体共聚焦显微镜检查，我们结合组织病理学确定了睑缘的不同层次、表皮的不同层次和皮肤附属器。
该研究概述了与标准组织学检查相比活体共聚焦显微镜下人类睑缘的特征，并证实活体CLSM无法观察到睑板腺腺泡结构。
