Ministry of Education Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
Mikrochim Acta. 2020 Jul 16;187(8):451. doi: 10.1007/s00604-020-04441-1.
A method has been developed for preparation of surface molecularly imprinted polymer functionalized silica nanoparticles (SiO@MPS@MIP). Firstly, the silica nanoparticles are prepared by a one-pot sol-gel method using tetraethylorthosilicate and 3-methacryloxypropyltrimethoxysilane as functional monomers. Next, the template molecule (L-Trp) is self-assembled with the functional monomer (acrylamide). Finally, SiO@MPS@MIP are prepared using N,N'-methylenebisacrylamide as the cross-linker. The prepared SiO@MPS@MIP have an average diameter of about 6.3 ± 1.2 nm. They exhibit good selectivity toward L-Trp with an imprinting factor of 6.3. The adsorption isotherm data was well described by the Langmuir model. The maximum adsorption capacities of SiO@MPS@MIP for L-Trp and D-Trp were calculated to be 11.1 ± 0.9 and 2.66 ± 0.16 mg g, respectively. An enantiomer excess value of 100% was achieved after adsorption of racemic Trp by the material. The work suggests that SiO@MPS@MIP are a promising material for enantioseparation of Trp racemate in aqueous media. Graphical abstract.
一种用于制备表面分子印迹聚合物功能化硅纳米粒子 (SiO@MPS@MIP) 的方法已经开发出来。首先，通过使用四乙氧基硅烷和 3-甲基丙烯酰氧基丙基三甲氧基硅烷作为功能单体的一锅溶胶-凝胶法制备硅纳米粒子。接下来，模板分子 (L-Trp) 与功能单体 (丙烯酰胺) 自组装。最后，使用 N,N'-亚甲基双丙烯酰胺作为交联剂制备 SiO@MPS@MIP。制备的 SiO@MPS@MIP 的平均直径约为 6.3±1.2nm。它们对 L-Trp 表现出良好的选择性，印迹因子为 6.3。吸附等温线数据很好地用朗缪尔模型描述。SiO@MPS@MIP 对 L-Trp 和 D-Trp 的最大吸附容量分别计算为 11.1±0.9 和 2.66±0.16mg/g。通过该材料吸附外消旋 Trp 后，达到了 100%的对映体过量值。这项工作表明，SiO@MPS@MIP 是一种在水介质中拆分 Trp 外消旋体的有前途的材料。
