Qin Lei, He Xi-Wen, Li Wen-You, Zhang Yu-Kui
Department of Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, China.
J Chromatogr A. 2008 Apr 11;1187(1-2):94-102. doi: 10.1016/j.chroma.2008.02.004. Epub 2008 Feb 8.
A novel molecularly imprinted polymer (MIP) selective for tryptophan (Trp) was described where polymerization was performed in aqueous media. Three kinds of molecularly imprinted polymers were prepared with surface molecular imprinting technique on functionalized silica gel (F-silica gel). MIPs prepared using bonded beta-cyclodextrin (beta-CD) and acrylamide (AA), either separately or in combination have shown various recognition properties. The results of adsorption experiments indicated that the selectivity of MIP, which was synthesized with bonded beta-CD and AA [MIP(1)], was superior to those obtained with AA [MIP(2)] or bonded beta-CD [MIP(3)]. In addition, the high-performance liquid chromatography (HPLC) column packed with MIP(1) could not only separate Trp from other aromatic amino acids, but also separate the template from its enantiomer in aqueous mobile phase. This study developed a new method for chiral amino acid separation and purification.
描述了一种对色氨酸（Trp）具有选择性的新型分子印迹聚合物（MIP），其聚合反应在水性介质中进行。采用表面分子印迹技术在功能化硅胶（F-硅胶）上制备了三种分子印迹聚合物。使用键合β-环糊精（β-CD）和丙烯酰胺（AA）单独或组合制备的MIP表现出各种识别特性。吸附实验结果表明，用键合β-CD和AA合成的MIP [MIP(1)] 的选择性优于用AA [MIP(2)] 或键合β-CD [MIP(3)] 获得的选择性。此外，填充有MIP(1)的高效液相色谱（HPLC）柱不仅可以将Trp与其他芳香族氨基酸分离，还可以在水性流动相中从其对映体中分离模板。本研究开发了一种手性氨基酸分离和纯化的新方法。
